Effects of L-glutamine on boar sperm quality during liquid storage at 17°C

Animal Reproduction Science
Shunwei WangJiabo Zhou

Abstract

The quality of boar spermatozoa is affected by oxidative stress during preservation in vitro. It has been demonstrated that L-Glutamine (Gln) can effectively protect cells from oxidative stress-induced injury. There are, however, no reports to date evaluating the effects of Gln on boar semen liquid preservation at 17 °C. The aims of the present study were to elucidate whether the addition of Gln to the extender BTS could improve the quality of boar spermatozoa when stored at 17 °C and to determine the mechanism underlying Gln protection of spermatozoa against preservation-induced damage. Boar semen samples were collected and diluted with Beltsville Thawing Solution (BTS) containing different concentrations (0, 10, 20, 40 or 80 mM) of Gln. The results indicated the addition of 20 mM Gln to the BTS improved (P < 0.05) the motility, acrosome integrity and membrane integrity of boar sperm during liquid preservation. Interestingly, treatment of spermatozoa with Gln addition to the extender resulted in ROS quenching, while enhancing γ-glutamyl cysteine synthetase (γ-GCS) activity, and glutathione (GSH) content of spermatozoa. These results suggest that BTS supplemented with Gln can provide greater protective capacity to boar sperm ag...Continue Reading

Citations

Aug 6, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Tian-Yu FengJian-Hong Hu
May 18, 2019·Reproduction in Domestic Animals = Zuchthygiene·Na WangJia-Bo Zhou
Jun 3, 2020·Zygote : the Biology of Gametes and Early Embryos·Linlin SunWenxian Zeng
Oct 11, 2019·Dose-response : a Publication of International Hormesis Society·Lei Wang, Lili Zhuang
Aug 7, 2021·Antioxidants·Miguel A SilvestreMaría Castelló-Ruiz

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