PMID: 8958148Jan 1, 1997Paper

Effects of lazaroids and a peroxynitrite scavenger in a cell model of peroxynitrite toxicity

Free Radical Biology & Medicine
G J FiciP F VonVoigtlander

Abstract

We developed a cerebellar granule cell model of peroxynitrite toxicity and showed that certain sulfhydryl-containing compounds (e.g., penicillamine) present as concurrent treatments could inhibit this toxicity. In the present study, 21-aminosteroid and pyrrolopyrimidine lazaroids were tested for cytoprotection in this peroxynitrite toxicity model. In addition, we tested for added protection using a peroxynitrite scavenger concurrent treatment combined with a lazaroid post-treatment. The toxicity assay utilized cells that were previously exposed to 100 microM L-buthionine (S,R)-sulfoximine (BSO), an inhibitor of gamma-glutamyl-cysteine synthetase, for 24 h. This sublethal concentration of BSO shifted the peroxynitrite (1-1000 microM) toxicity curve to the left by more than one-half of a log unit. The half-maximal toxicity concentration (TC50) of peroxynitrite in cells treated with BSO was 50 microM. The 21-aminosteroids, U-74006F and U-74500A, and the pyrrolopyrimidines, U-91736B and U-101033E, were tested as post-treatments. U-74006F and U-74500A had EC50 values of approximately 100 microM (concentrations which blocked 50% of the toxicity). U-91736B and U-101033E had EC50 values of 1 microM and showed 100% protection at 3-10 mi...Continue Reading

References

Nov 1, 1992·Chemical Research in Toxicology·W H KoppenolJ S Beckman
Aug 1, 1991·Archives of Biochemistry and Biophysics·R RadiB A Freeman
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Jan 1, 1989·Free Radical Biology & Medicine·J M Braughler, J F Pregenzer
Feb 1, 1995·Free Radical Biology & Medicine·S M VroegopS E Buxser
Jan 1, 1994·Methods in Enzymology·J S BeckmanJ P Crow
Aug 17, 1994·Biochemical Pharmacology·R S Fernandes, T G Cotter
Jan 1, 1996·Acta Neurochirurgica. Supplement·E D HallG L Bundy

❮ Previous
Next ❯

Citations

Jul 12, 2002·Phytotherapy Research : PTR·Hye Rhi ChoiHae Young Chung
Mar 17, 2010·Pflügers Archiv : European journal of physiology·Anthony Lau, Michael Tymianski
Aug 23, 2002·European Journal of Pharmacology·Vadim V SumbayevBernhard Brüne
Sep 3, 1998·European Journal of Pharmacology·T T Rohn, M T Quinn
Aug 12, 1999·European Journal of Pharmacology·J C StocletA L Kleschyov
Apr 29, 1998·Pharmacology & Toxicology·A T DemiryürekI Kanzik
Jun 6, 2006·Medicinal Research Reviews·Ana OlmosSalvador Máñez
Sep 2, 2004·Comparative Hepatology·Gordana KocicDusan Sokolovic
Sep 18, 2012·Journal of the American Chemical Society·Quanjuan ZhangYoujun Yang
Jun 19, 1998·Neurosurgery·R L MacdonaldP K Andrus
Aug 13, 1999·Journal of Applied Physiology·G SupinskiA DiMarco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.