PMID: 7516817Feb 1, 1994Paper

Effects of lead on osteoclast-like cell formation in mouse bone marrow cell cultures

Calcified Tissue International
T MiyaharaK Yokoyama

Abstract

To examine an effect of lead (Pb) on the process of osteoclast-like cell formation from its progenitors, we used a mouse bone marrow culture system in which osteoclast-like multinucleated cells (MNCs) were formed in response to bone-resorbing agents. In a 9-day culture period, Pb dose-dependently stimulated MNC formation over the concentration range 2-10 microM, whereas at 40 microM Pb, MNC formation declined. In an 11-day culture period, MNC formation reached a maximum at 5 microM Pb and decreased with increasing concentration of Pb at 10-40 microM. Pb-stimulated MNC formation was inhibited by both indomethacin and SC19220, an antagonist of prostaglandin E2 (PGE2) receptor. Pb stimulated the production of PGE2 in marrow cell cultures, suggesting that Pb-stimulated MNC formation is dependent on the production of PGE2. 3-Isobutyl-1-methylxanthine potentiated Pb-stimulated MNC formation and 2',5'-dideoxyadenosine, an inhibitor of adenylate cyclase, inhibited it. A calcium ionophore A23187 increased Pb-induced MNC formation and verapamil, a calcium channel blocker, depressed it. It is possible that a PGE2-induced increase in the levels of cyclic adenosine 3',5'-monophosphate (cAMP) and calcium ions in marrow cells is involved in P...Continue Reading

References

Dec 1, 1990·Journal of Cellular Physiology·T YamashitaT Suda
Aug 1, 1991·Bulletin of Environmental Contamination and Toxicology·T MiyaharaS Kuze
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F A SchanneJ F Rosen
Jul 11, 1989·Biochimica Et Biophysica Acta·Y SuzukiS Murota
Feb 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T AkatsuT Suda
Nov 1, 1988·Endocrinology·N TakahashiT Suda

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Citations

Mar 5, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M E RuppelD B Burr
Aug 26, 2006·Journal of Materials Science. Materials in Medicine·Chia-Tze KaoTsui-Hsien Huang
Oct 30, 2015·Clinical Orthopaedics and Related Research·Caleb BehrendEdward Puzas
Aug 29, 2013·Clinical Orthopaedics and Related Research·John T RiehlKenneth J Koval
Dec 12, 2018·The Journal of Veterinary Medical Science·Genya ShimboKazuro Miyahara
Jun 1, 1997·Clinical Orthopaedics and Related Research·J Y WangD T Tsukayama
Mar 28, 2020·International Journal of Molecular Sciences·Emilia MetrykaIrena Baranowska-Bosiacka
May 1, 1997·Journal of Biomedical Materials Research·K G Nichols, D A Puleo

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