PMID: 9430617Feb 28, 1998Paper

Effects of lens major intrinsic protein on glycerol permeability and metabolism

The Journal of Membrane Biology
C KushmerickR T Mathias

Abstract

Lens Major Intrinsic Protein (MIP) is a member of a family of membrane transport proteins including the Aquaporins and bacterial glycerol transporters. When expressed in Xenopus oocytes, MIP increased both glycerol permeability and the activity of glycerol kinase. Glycerol permeability (pGly) was 2.3 +/- 0.23 x 10(-6) cm sec-1 with MIP vs. 0.92 +/- 0.086 x 10(-6) cm sec-1 in control oocytes. The pGly of MIP was independent of concentration from 5 x 10(-5) to 5 x 10(-2) m, had a low temperature dependence, and was inhibited approximately 90%, 80% and 50% by 1.0 mM Hg++, 0.2 mM DIDS (diisothiocyanodisulfonic stilbene), and 0.1 mm Cu++, respectively. MIP-enhanced glycerol phosphorylation, resulting in increased incorporation of glycerol into lipids. This could arise from an increase in the total activity of glycerol kinase, or from an increase in its affinity for glycerol. Based on methods we present to distinguish these mechanisms, MIP increased the maximum rate of phosphorylation by glycerol kinase (0.12 +/- 0.03 vs. 0.06 +/- 0.01 pmol min-1 cell-1) without changing the binding of glycerol to the kinase (KM approximately 10 micron).

Citations

Aug 28, 1998·Current Opinion in Cell Biology·P M Deen, C H van Os
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·William E C HarriesRobert M Stroud
Oct 28, 2017·International Journal of Molecular Sciences·Jennifer Virginia Roche, Susanna Törnroth-Horsefield
Mar 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kulandaiappan VaradarajRichard T Mathias
Aug 23, 2002·The Journal of Biological Chemistry·Leila V VirkkiWalter F Boron
Jul 10, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·P DonaldsonR T Mathias
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L V VirkkiW F Boron
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·J. Bernard Heymann, Andreas Engel

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