Effects of leukotriene C4 and D4, histamine and bradykinin on cytosolic calcium concentrations and adhesiveness of endothelial cells and neutrophils

Clinical and Experimental Immunology
M Heimbürger, J Palmblad

Abstract

We compared the effects of leukotrienes B4, C4 and D4 (LTB4, C4 and D4) in vitro, as well as of histamine and bradykinin, on adhesive interactions between cultured umbilical vein endothelial cells (HUVEC) and polymorphonuclear neutrophils (PMN) and on cytosolic calcium transients, [Ca(2+)](i), in vitro. LTB4, but not LTC4 or LTD4 (at 1-100 nM), increased HUVEC adhesiveness for PMN, maximally 2.8 fold; in addition, PMN adhesion was augmented by LTB4 (but not by LTC4 and LTD4) to a plastic surface. Rapid, but smaller increments of HUVEC (but not of PMN) adhesiveness were induced by histamine and bradykinin (at 10 microM). Nonetheless, LTC4 and LTD4 (at 100 nm) induced rapid rises of [Ca(2+)](i) in HUVEC, whereas approximately 100-fold higher concentrations were needed of histamine and bradykinin for similar rises. In PMN LTD4 (and LTB4) induced rapid increases of [Ca(2+)](i), whereas no significant effect was seen with LTC4, histamine or bradykinin. The [Ca(2+)](i) responses to LTC4 and LTD4 were inhibited by the peptidoleukotriene receptor blocker SKF 104,353. Thus, LTB4 and the peptidoleukotrienes display disparate profiles as inducers of adhesion and calcium transients in PMN and HUVEC, indicating discrete differences in the s...Continue Reading

References

Nov 1, 1992·Clinical and Experimental Immunology·J E Palmblad, R Lerner
Aug 2, 1990·Nature·T A Springer
Jan 30, 1990·Biochemical and Biophysical Research Communications·J PalmbladR Lerner
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M McIntyreS M Prescott
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Samuelsson
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L HooverR A Lewis
Feb 1, 1984·Acta Ophthalmologica·J StjernschantzM Sears

❮ Previous
Next ❯

Citations

Mar 20, 2001·Infection and Immunity·P MancusoM Peters-Golden
May 8, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L FregoneseG A Rossi
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rainer SpanbroekAndreas J R Habenicht
May 21, 2008·Journal of Negative Results in Biomedicine·David W ReidE Haydn Walters
Nov 7, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H MitaK Akiyama
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H ShirasakiT Himi
Jan 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonio Di GennaroJesper Z Haeggström
Oct 16, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z Diamant, A P Sampson
Apr 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hong QiuJesper Z Haeggström
Nov 29, 2008·Journal of Leukocyte Biology·Cornelia M BertramKanti D Bhoola
Aug 16, 2014·Scandinavian Journal of Immunology·P AnderssonJ Palmblad
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·S T Holgate, A P Sampson
Sep 17, 2003·Journal of Internal Medicine·C J TreutigerJ E W Palmblad
Jan 15, 2013·American Journal of Obstetrics and Gynecology·Kuen-Bao ChenChi-Yuan Li
Feb 11, 1999·Biochemical Pharmacology·M HeimbürgerJ Palmblad
Apr 7, 2009·Life Sciences·Anne-Sofie Johansson, Jan Palmblad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.