Abstract
We compared the effects of leukotrienes B4, C4 and D4 (LTB4, C4 and D4) in vitro, as well as of histamine and bradykinin, on adhesive interactions between cultured umbilical vein endothelial cells (HUVEC) and polymorphonuclear neutrophils (PMN) and on cytosolic calcium transients, [Ca(2+)](i), in vitro. LTB4, but not LTC4 or LTD4 (at 1-100 nM), increased HUVEC adhesiveness for PMN, maximally 2.8 fold; in addition, PMN adhesion was augmented by LTB4 (but not by LTC4 and LTD4) to a plastic surface. Rapid, but smaller increments of HUVEC (but not of PMN) adhesiveness were induced by histamine and bradykinin (at 10 microM). Nonetheless, LTC4 and LTD4 (at 100 nm) induced rapid rises of [Ca(2+)](i) in HUVEC, whereas approximately 100-fold higher concentrations were needed of histamine and bradykinin for similar rises. In PMN LTD4 (and LTB4) induced rapid increases of [Ca(2+)](i), whereas no significant effect was seen with LTC4, histamine or bradykinin. The [Ca(2+)](i) responses to LTC4 and LTD4 were inhibited by the peptidoleukotriene receptor blocker SKF 104,353. Thus, LTB4 and the peptidoleukotrienes display disparate profiles as inducers of adhesion and calcium transients in PMN and HUVEC, indicating discrete differences in the s...Continue Reading
References
Nov 1, 1992·Clinical and Experimental Immunology·J E Palmblad, R Lerner
Nov 1, 1992·The Journal of Cell Biology·Y SugamaA B Malik
Jun 1, 1990·Scandinavian Journal of Immunology·P LindströmM Patarroyo
Aug 2, 1990·Nature·T A Springer
Feb 1, 1990·The Journal of Cell Biology·G A ZimmermanS M Prescott
Jan 30, 1990·Biochemical and Biophysical Research Communications·J PalmbladR Lerner
Jan 2, 1987·European Journal of Biochemistry·P D LewT Pozzan
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M McIntyreS M Prescott
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Samuelsson
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L HooverR A Lewis
Feb 1, 1984·Acta Ophthalmologica·J StjernschantzM Sears
Citations
Mar 20, 2001·Infection and Immunity·P MancusoM Peters-Golden
May 8, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L FregoneseG A Rossi
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rainer SpanbroekAndreas J R Habenicht
May 21, 2008·Journal of Negative Results in Biomedicine·David W ReidE Haydn Walters
Jul 13, 2000·Cell Motility and the Cytoskeleton·J H WangF C Yin
Nov 7, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H MitaK Akiyama
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H ShirasakiT Himi
Apr 27, 2001·European Journal of Biochemistry·M SjöströmJ Z Haeggström
Jan 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonio Di GennaroJesper Z Haeggström
May 27, 2008·British Journal of Pharmacology·A-S JohanssonJ Palmblad
Oct 16, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Z Diamant, A P Sampson
Feb 26, 2000·Clinical and Experimental Immunology·M HeimbürgerJ Bratt
Jul 21, 2011·Pharmacological Reviews·Magnus BäckG Enrico Rovati
Apr 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hong QiuJesper Z Haeggström
Nov 29, 2008·Journal of Leukocyte Biology·Cornelia M BertramKanti D Bhoola
Aug 16, 2014·Scandinavian Journal of Immunology·P AnderssonJ Palmblad
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·S T Holgate, A P Sampson
Sep 17, 2003·Journal of Internal Medicine·C J TreutigerJ E W Palmblad
Jan 15, 2013·American Journal of Obstetrics and Gynecology·Kuen-Bao ChenChi-Yuan Li
Aug 26, 1998·Biochemical and Biophysical Research Communications·M Heimbürger, J Palmblad
Feb 11, 1999·Biochemical Pharmacology·M HeimbürgerJ Palmblad
Apr 7, 2009·Life Sciences·Anne-Sofie Johansson, Jan Palmblad