PMID: 9000645Jan 1, 1997Paper

Effects of licorice derivatives on vascular smooth muscle function

Life Sciences
Andrew S BremD J Morris

Abstract

Vascular smooth muscle contains a bidirectional form of the enzyme 11beta-hydroxysteroid dehydrogenase (11beta-HSD) which can inactivate (dehydrogenase) endogenous circulating glucocorticoids (GCs) or activate (oxo-reductase) 11-dehydro-metabolites by their conversion back to the parent steroid. Enzyme direction in vascular smooth muscle (VSM) has potential physiological consequences since GCs may enhance the response to known vasoconstrictors. We determined that carbenoxolone is a competitive inhibitor of 11beta-HSD contained in VSM cells with a lower Ki for forward dehydrogenase reaction (0.02 microM) compared to the oxo-reductase reaction (0.41 microM). To test whether changes in enzyme directional activity can affect the contractile response, aortae from adrenally intact Sprague Dawley rats were removed and sectioned into 2.5 mm rings. Rings were incubated with corticosterone 10 nM plus carbenoxolone (CBX) 10 microM (a concentration well above the Ki for both the dehydrogenase and oxo-reductase reaction) for 24 hrs. These rings showed an enhanced dose dependent contractile response to phenylephrine (PE) 0.01 microM(-1) microM and to angiotensin II 1 microM compared to rings incubated with corticosterone alone, CBX alone, or...Continue Reading

Citations

Mar 31, 1998·Life Sciences·R B BinaA S Brem
Jun 5, 2003·Molecular and Cellular Endocrinology·David J MorrisMatthew P Hardy
Nov 10, 2001·Trends in Endocrinology and Metabolism : TEM·T C Sandeep, B R Walker
Aug 13, 2003·Kidney International·Bettina LanzMarkus G Mohaupt
Feb 24, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·H HatakeyamaI Miyamori
Nov 7, 2007·European Journal of Endocrinology·Brian R Walker
Jun 3, 2010·Clinical Science·Piotr SobieszczykJoshua A Beckman
May 28, 2004·Metabolism: Clinical and Experimental·David J MorrisAndrew S Brem
Oct 28, 2005·Journal of the American Society of Nephrology : JASN·Bert KadereitMarkus G Mohaupt
Jan 18, 2002·Endocrinology·Gui-Min WangMatthew P Hardy
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Daliao XiaoLubo Zhang
Jan 18, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Daliao XiaoLubo Zhang
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Kestrel M RogersShumei Yang
Dec 26, 2001·Journal of the American Society of Nephrology : JASN·Andreas SerraBruno Vogt
Jan 3, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·A S Brem

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