Effects of ligand binding on the association properties and conformation in solution of retinoic acid receptors RXR and RAR

Journal of Molecular Biology
P F EgeaD Moras

Abstract

In higher eukaryotes, vitamin A derived metabolites such as 9-cis and all-trans retinoic acid (RA), are involved in the regulation of several essential physiological processes. Their pleiotropic physiological effects are mediated through direct binding to cognate nuclear receptors RXRs and RARs that act as regulated transcription factors belonging to the superfamily of nuclear hormone receptors. Hormone binding to the structurally conserved ligand-binding domain (LBD) of these receptors triggers a conformational change that principally affects the conserved C-terminal transactivation helix H12 involved in transcriptional activation. We report an extensive biophysical solution study of RAR alpha, RXR alpha LBDs and their corresponding RXR alpha/RAR alpha LBD heterodimers combining analytical ultracentrifugation (AUC), small-angle X-ray and neutron scattering (SAXS and SANS) and ab initio three-dimensional shape reconstruction at low resolution. We show that the crystal structures of RXRs and RARs LBDs correlate well with the average conformations observed in solution. Furthermore we demonstrate the effects of 9-cisRA and all-transRA binding on the association properties and conformations of RXR alpha and RAR alpha LBDs in soluti...Continue Reading

References

Dec 14, 1995·Nature·R L WagnerR J Fletterick
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KerstenN Noy
Nov 15, 1994·Biochemistry·J C HansenB Demeler
Aug 1, 1994·Biophysical Journal·J Garcia de la TorreJ J Lopez Cascales
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AllenbyP Chambon
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Jan 1, 1996·Nature Structural Biology·J M WurtzH Gronemeyer
Feb 1, 1996·Nature Structural Biology·J M WurtzH Gronemeyer
Feb 1, 1997·Current Opinion in Biotechnology·W F Stafford
Oct 24, 1997·Nature·A M BrzozowskiM Carlquist
Feb 6, 1998·Journal of Molecular Biology·Z P ChenH Gronemeyer
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
Mar 21, 1998·Nature Structural Biology·B P KlaholzD Moras
Mar 26, 1998·International Journal of Biological Macromolecules·S J PerkinsD Chamberlain
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D M TanenbaumP B Sigler
Jun 10, 1998·Nature·S P Williams, P B Sigler
Jun 26, 1998·Current Opinion in Cell Biology·D Moras, H Gronemeyer
Aug 24, 1999·Molecular and Cellular Biology·J ZhangM A Lazar
Feb 19, 2000·Current Opinion in Biotechnology·O Byron, R J Gilbert
Jul 6, 2000·FEBS Letters·P F EgeaD Moras

❮ Previous
Next ❯

Citations

Dec 15, 2005·The British Journal of Nutrition·Laure VilleneuveChantal L Cahu
Feb 8, 2007·Annual Review of Biophysics and Biomolecular Structure·Jan Lipfert, Sebastian Doniach
Jul 27, 2002·Annual Review of Pharmacology and Toxicology·Li-Na Wei
Feb 16, 2005·Annual Review of Physiology·Kendall W Nettles, Geoffrey L Greene
Nov 6, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Yusheng WangChunyu Zeng
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yan ChenJoachim D Müller
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Emilie GaillardCécile Rochette-Egly
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gaétan BourCécile Rochette-Egly
Oct 16, 2015·Canadian Journal of Physiology and Pharmacology·Madina IskakovaCécile Rochette-Egly
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hai-Chon Lee, Steven F Ziegler
Jun 20, 2006·Journal of Molecular Biology·Alessandro S NascimentoIgor Polikarpov
Nov 21, 2009·Journal of Molecular Biology·Mona AliSebastian Doniach
May 17, 2006·Trends in Ecology & Evolution·Richard C Hartley, Malcolm W Kennedy
Jul 27, 2004·Protein Science : a Publication of the Protein Society·Mark E HarderMichael I Schimerlik
May 28, 2009·Nuclear Receptor Signaling·Cécile Rochette-Egly, Pierre Germain
Oct 29, 2017·Journal of the American Society of Nephrology : JASN·Amelie T van der VenFriedhelm Hildebrandt
Dec 21, 2004·Cell Death and Differentiation·A SzantoL Nagy
May 1, 2002·Molecular Endocrinology·Pascal F EgeaDino Moras
Aug 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jian-guang WangLingtao Wu
Jun 7, 2011·Biochemistry. Biokhimii︠a︡·I V PerevoshchikovaY N Antonenko
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·R I ZhdanovG Bischoff
Feb 28, 2003·The Journal of Biological Chemistry·Kristen A JohnsonRobert A Terkeltaub
Nov 24, 2011·Journal of Computational Chemistry·Xue LiKenneth M Merz
Dec 12, 2002·Multiple Sclerosis : Clinical and Laboratory Research·W RoyalC D Gajewski
Oct 10, 2001·Biochimica Et Biophysica Acta·R Benezra
May 18, 2021·Frontiers in Genetics·Meimiao FangLiang Chen
Mar 17, 2004·Gene·Julie Bastien, Cécile Rochette-Egly
Aug 9, 2005·Journal of the American Society for Mass Spectrometry·Johan LengqvistWilliam J Griffiths
Feb 27, 2007·The Journal of Nutritional Biochemistry·Mary M McGrane
Jun 7, 2011·Journal of Chemical Information and Modeling·Aqeel AhmedHolger Gohlke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.