PMID: 9449128Feb 4, 1998Paper

Effects of ligation and reperfusion of hepatic afferent vessels on the composition of liver cell membrane in the rat: 1H- and 31P-magnetic resonance spectroscopic analysis

NMR in Biomedicine
Y HayakawaN Yasuda

Abstract

Biochemical changes that occur within hepatic tissue of the rat during ischemia and subsequent reperfusion were investigated using magnetic resonance spectroscopy of liver extracts. Hepatic ischemia was produced in the rat by a continuous clamping of the left branches of the hepatic artery and portal vein. In the reperfusion experiments, the vascular clamps were released after 30 or 120 min of ischemic periods. At the end of the periods of ischemia and/or reperfusion, the left and middle hepatic lobes were dissected and processed for subsequent 1H-MRS and 31P-MRS analyses. Phosphatidylcholine, sphingomyelin, phosphatidic acid and phosphatidylethanolamine contents all showed reduction of about 30% after 120 min of ischemia. In contrast, the content of lysophosphatidylcholine showed relatively small changes following ischemia. Ten minutes after initiation of reperfusion, further decline of the total phospholipid content resulting in as much 42% reduction was observed. Then it recovered to nearly the control level when ischemia was for 30 min, but to only 65% of the control level when ischemia was for 120 min. The cholesterol/-N-(CH3)3 ratio, generally regarded as a parameter for membrane fluidity, showed about a 40% increase when...Continue Reading

References

Jul 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H T EdzesJ Valk
Apr 1, 1991·The American Journal of Physiology·R T Worrell, R A Frizzell
Mar 1, 1991·The American Journal of Physiology·M M BersohnR S Weiss
Apr 1, 1989·Analytical Biochemistry·M L SparlingS I Chan
Jun 19, 1986·Biochimica Et Biophysica Acta·T W FanO Jardetzky
Mar 21, 1986·Biochimica Et Biophysica Acta·A Reuben, R M Allen
Oct 15, 1984·Biochemical and Biophysical Research Communications·W B WeglickiI T Mak
Apr 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H HanaokaN Yasuda

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Citations

Dec 28, 1999·American Journal of Ophthalmology·K Ohtsuka, M Hashimoto
Jul 23, 2005·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Yoshichika YoshiokaManabu Kubokawa
Feb 22, 2002·The Journal of Surgical Research·Rocco RicciardiWilliam C Meyers

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