PMID: 8942398Nov 1, 1996Paper

Effects of limb immobilization on cytochrome c oxidase activity and GLUT4 and GLUT5 protein expression in human skeletal muscle

Clinical Science
S J BlakemoreH S Hundal

Abstract

1. We investigated the effects of limb immobilization (for 1 or 6 weeks) in a long leg cast after a closed tibial fracture (n = 11). Biopsies of vastus lateralis were taken on admission and after either 1 week (n = 5) or 6 weeks (n = 6) and analysed for muscle fibre type characteristics, cytochrome c oxidase activity and the abundance of GLUT4 and GLUT5 hexose transporters. 2. After 1 week of immobilization there was a significant decrease (8%) in the cross-sectional area of type I, but not type II, muscle fibers and in the protein-DNA ratio (16%) compared with the initial biopsy. Six weeks of immobilization led to further muscle atrophy compared with the initial biopsy and a further reduction in the cross-sectional area of both type I and II fibres (29% and 36% decrease respectively) and in the protein-DNA ratio (25%). No changes were observed in the free leg after 1 week. However, at th end of the 6 week study period, the cross-sectional area of boty type I and II fibres of the free leg were increased (7% and 5%) and there was significant increase in the protein-DNA ratio (14%), indicating a net increase in muscle protein content. 3. Assay for cytochrome c oxidase activity showed significant reduction after 1 (30%) or 6 weeks...Continue Reading

Citations

Jan 19, 2006·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·R L RosalesD Dressler
Mar 5, 2014·Muscle & Nerve·Christopher C MatthewsPaul S Fishman
Apr 4, 2007·Cell Metabolism·Shaohui Huang, Michael P Czech
Aug 3, 2013·European Journal of Sport Science·Martin GramFlemming Dela
Oct 15, 2014·BioMed Research International·Martin FlückThomas Gustafsson
Apr 5, 2013·Nutrition Reviews·Benjamin T Wall, Luc J C van Loon
Oct 2, 2003·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Anne-Caroline Dupont SalterGerald E Loeb
May 23, 2007·NMR in Biomedicine·Neeti PathareGlenn A Walter
May 9, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Darren E R WarburtonMark J Haykowsky
Apr 10, 2008·American Journal of Physiology. Endocrinology and Metabolism·Veronique Douard, Ronaldo P Ferraris
Feb 6, 2010·Critical Care Medicine·Michael J Rennie
Jul 23, 2002·Journal of Applied Physiology·G Lynis Dohm
Jan 17, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M GasterH D Schrøder
Nov 6, 2003·Journal of Applied Physiology·Gregory R AdamsKenneth M Baldwin
Sep 25, 2003·Metabolism: Clinical and Experimental·Kay OhlendieckVictor R Preedy

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