PMID: 7150567Dec 7, 1982Paper

Effects of limited tryptic proteolysis of bovine neurophysins on molecular properties of hormone binding, self-association, and antigenicity

Biochemistry
D M AbercrombieI M Chaiken

Abstract

Limited tryptic fragmentation of disulfide-intact bovine neurophysins I and II (NP-I and -II, respectively) has been found to cause selective disruption of both hormone binding and neurophysin self-association. Loss of binding interactions, measured as a loss of ability to stimulate retardation of 125I-labeled neurophysin on Met-Tyr-Phe-amino-butylaminoagarose, is complete within 3 h at 37 degrees C. Reverse-phase high-performance liquid chromatography (HPLC) analysis of tryptic digests of neurophysin I allows detection of two major protein products and the peptide fragment 1-8. Release of the latter N-terminal piece occurs at about the same rate as loss of binding interactions. Reverse-phase HPLC elution behavior before and after performic acid oxidation and amino acid composition of the protein products led to their identification as NP-I-(9-93) (the 9-93 sequence) and [des-19,20]NP-I-(9-93) (the 9-93 sequence with the dipeptide 19-20 missing) for the more rapidly and more slowly formed species, respectively. NP-I-(9-93), unlike intact neurophysin I, is not retarded strongly by either Met-Tyr-Phe-amino-butylaminoagarose or neurophysin II-Sepharose. In contrast, both NP-I-(9-93) and [des-19,20]NP-I-(9-93) are equally as effect...Continue Reading

References

Nov 1, 1979·Angewandte Chemie·R Acher
Jan 1, 1979·Annual Review of Biochemistry·E Breslow
May 1, 1977·The Journal of Cell Biology·H GainerM J Brownstein
Feb 21, 1975·Annals of the New York Academy of Sciences·I M ChaikenH C Taylor
Feb 21, 1975·Annals of the New York Academy of Sciences·J J Dreifuss
Jan 26, 1976·Biochemical and Biophysical Research Communications·T C Wuu, S E Crumm
Jan 25, 1980·Science·M J BrownsteinH Gainer
Jan 1, 1981·CRC Critical Reviews in Biochemistry·I M Chaiken
May 14, 1982·Biochemical and Biophysical Research Communications·E BreslowR T Co
Apr 15, 1980·Archives of Biochemistry and Biophysics·R Tellam, D J Winzor

Citations

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S BurmanE Breslow
Nov 1, 1991·International Journal of Peptide and Protein Research·C A HasselbacherW R Laws

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