PMID: 6409153Jul 29, 1983Paper

Effects of lipid peroxidation on adrenal microsomal monooxygenases

Biochimica Et Biophysica Acta
W C BroganH D Colby

Abstract

Incubation of guinea pig adrenal microsomes with 10(-6) M ferrous (Fe2+) ion and adrenal cytosol initiated high levels of lipid peroxidation as measured by the production of malonaldehyde. Cytosol or Fe2+ alone had little effect on microsomal malonaldehyde formation. When microsomes were incubated in the presence of Fe2+ and cytosol, malonaldehyde levels continued to increase for at least 60 min. Accompanying the lipid peroxidation was a decline in adrenal microsomal monooxygenase activities. The rates of metabolism of xenobiotics (benzphetamine demethylase, benzo[a]pyrene hydroxylase) as well as steroids (21-hydroxylation) decreased as malonaldehyde levels increased. In addition, cytochrome P-450 levels, NADPH- and NADH-cytochrome c reductase activities, and substrate interactions with cytochrome(s) P-450 decreased as lipid peroxidation progressed. Inhibition of lipid peroxidation by increasing microsomal protein concentrations during the incubation period prevented the changes in microsomal metabolism. Malonaldehyde had no direct effects on adrenal microsomal enzyme activities. The results indicate that lipid peroxidation may have significant effects on adrenocortical function, diminishing the capacity for both xenobiotic and...Continue Reading

References

Sep 30, 1975·Annals of the New York Academy of Sciences·A E Kitabchi, W H West
Jan 1, 1976·Annual Review of Pharmacology and Toxicology·G L Plaa, H Witschi
Sep 30, 1975·Annals of the New York Academy of Sciences·D Hornig
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Jan 26, 1981·Biochimica Et Biophysica Acta·W C BroganH D Colby
Jun 1, 1980·Lipids·M K Logani, R E Davies
May 1, 1960·Archives of Biochemistry and Biophysics·H ZALKIN, A L TAPPEL

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Citations

Jan 1, 1988·Free Radical Biology & Medicine·J D Gower
Feb 1, 1995·Free Radical Biology & Medicine·H D ColbyM Levitt
Aug 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·J Klimek
May 24, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Ryszard MilczarekJerzy Klimek

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