Effects of lipopolysaccharide on glial phenotype and activity of glutamate transporters: Evidence for delayed up-regulation and redistribution of GLT-1

Neurochemistry International
R D O'SheaP M Beart

Abstract

Excitatory amino acid transporters (EAATs) are responsible for homeostasis of extracellular L-glutamate, and the glial transporters are functionally dominant. EAAT expression or function is altered in acute and chronic neurological conditions, but little is known about the regulation of EAATs in reactive astroglia found in such neuropathologies. These studies examined the effects of the bacterial endotoxin lipopolysaccharide (LPS) on glial EAATs in vitro. The effects of LPS (1 microg/ml, 24-72 h) on EAAT activity and expression were examined in primary cultures of mouse astrocytes. [(3)H]D-aspartate uptake increased to 129% of control by 72 h treatment with LPS. Saturation analysis revealed that apparent K(m) was unchanged whilst V(max) was significantly increased to 172% of control by 72 h LPS treatment. Biotinylation and Western blotting indicated that cell-surface expression of GLT-1 was significantly elevated (146% control) by LPS treatment whereas GLAST expression was unchanged. Confocal analyses revealed that LPS treatment resulted in cytoskeletal changes and stellation of astrocytes, with rearrangement of F-actin (as shown by phalloidin labelling). Immunocytochemistry revealed clustering of GLAST, and increased expressio...Continue Reading

References

Sep 25, 1997·Journal of Neuroscience Research·E M Abd-El-Basset, S Fedoroff
Aug 19, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D B StanimirovicA Muruganandam
May 23, 2001·Progress in Neurobiology·N C Danbolt
Dec 18, 2001·Brain Research. Developmental Brain Research·S FukamachiT Iwaki
Oct 9, 2002·Clinical and Experimental Pharmacology & Physiology·R D O'Shea
Aug 5, 2003·Glia·Christal C BowmanIan Marriott
Sep 6, 2003·Experimental Neurology·Maciej FigielJürgen Engele
Feb 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cristina RomeraMaria A Moro
Jul 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jianzheng Zhou, Margaret L Sutherland
Apr 23, 2005·Glia·Milos Pekny, Michael Nilsson
Jul 7, 2005·Journal of Neurochemistry·Céline VermeirenEmmanuel Hermans

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Citations

Mar 5, 2011·Amino Acids·Mikael Persson, Lars Rönnbäck
Mar 5, 2011·Amino Acids·Gabriel GrasAnne-Cécile Rimaniol
Oct 22, 2008·Molecular Neurobiology·Carole Escartin, Gilles Bonvento
Jul 5, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Xiu-Lan SunGang Hu
Dec 4, 2010·Neurochemical Research·Dorte M SkyttHelle S Waagepetersen
Apr 30, 2010·Animal Reproduction Science·Andrzej Przemysław Herman, Dorota Tomaszewska-Zaremba
May 12, 2009·Neurochemistry International·Sébastien TilleuxEmmanuel Hermans
May 23, 2007·Neurochemistry International·Amanda L Sheldon, Michael B Robinson
Nov 8, 2006·British Journal of Pharmacology·P M Beart, R D O'Shea
Mar 5, 2014·Journal of Neurochemistry·Chew L LauPhilip M Beart
Jun 10, 2014·Developmental Psychobiology·Gordon A Barr, Deirtra A Hunter
Jul 21, 2015·Cellular and Molecular Neurobiology·Arpita SharmaIshan K Patro
Mar 17, 2007·Neuroscience·J LipskiD Donnelly
Feb 15, 2015·Neuroscience Letters·Kazuyuki NakajimaShinichi Kohsaka
Nov 21, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ana P ArdaisLisiane O Porciúncula
May 15, 2007·Journal of Neuroscience Research·Sébastien Tilleux, Emmanuel Hermans
Mar 5, 2008·The Journal of Comparative Neurology·Tara M DesilvaPaul A Rosenberg
May 10, 2019·Frontiers in Physiology·Silvia PregnolatoKaren Luyt

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