Effects of lipopolysaccharide on leptin transport across the blood-brain barrier

Brain Research
Naoko NonakaWilliam A Banks

Abstract

Leptin is a 16-kDa protein secreted by fat cells and transported into the brain where it decreases appetite and increases body temperature. Leptin transport is saturable and regulated by epinephrine, triglycerides, and starvation. Lipopolysaccharide (LPS) is derived from bacterial cell walls and also decreases appetite and increases body temperature. LPS is known to increase leptin levels in serum and to affect the passage of other regulatory proteins across the blood-brain barrier (BBB). Here, we examined the ability of LPS, at doses which induce weight loss, to modify the BBB transport of radioactive leptin (I-Lep). The transport rate of intravenously injected I-Lep was decreased by 50-60% from 8 to 12 h after a single i.p. injection of LPS (3 mg/kg). The effect of LPS was dose-dependent. In comparison to the brain/serum ratio, the baseline cerebrospinal fluid (CSF)/serum ratio for I-Lep was much lower and not inhibited by LPS. LPS did not affect I-Lep transport when studied by the brain perfusion method nor was Ob-Ra mRNA expression in isolated brain microvessels altered, demonstrating that a circulating factor rather than altered BBB function was responsible for inhibition. Brain perfusion showed that LPS was not this facto...Continue Reading

References

Jul 28, 1995·Science·M A PelleymounterF Collins
Feb 1, 1996·The New England Journal of Medicine·R V ConsidineT L Bauer
May 1, 1996·The Journal of Clinical Investigation·C GrunfeldK R Feingold
Dec 1, 1996·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·L A CampfieldP Burn
Jan 5, 1999·Neuroendocrinology·R De Matteis, S Cinti
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·R FaggioniG Fantuzzi
Jun 23, 2000·The Journal of Clinical Investigation·K El-HaschimiJ S Flier
Apr 25, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A I IvanovA A Romanovsky
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F PicardY Deshaies
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C A MastronardiS M McCann
Jan 10, 2002·Brain, Behavior, and Immunity·S J Larson, A J Dunn
Apr 28, 2004·Diabetes·William A BanksJohn E Morley
Sep 11, 2004·Journal of Pineal Research·Jolanta JaworekWiesław W Pawlik

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Citations

Mar 19, 2013·Journal of Molecular Neuroscience : MN·Hung HsuchouWeihong Pan
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Weihong PanAbba J Kastin
Oct 21, 2006·Endocrine Journal·Hiroyuki ShimizuMasatomo Mori
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Czeslawa KowalBetty Diamond
Mar 31, 2009·Expert Opinion on Drug Delivery·J RipA G de Boer
Jun 25, 2009·Biochemical Pharmacology·Edson Lucas SantosJoão Bosco Pesquero
Feb 11, 2009·Cell Biochemistry and Function·Yusuf Ziya ZiylanRasim Mogulkoc
May 23, 2012·Annals of the New York Academy of Sciences·William A Banks
Mar 21, 2016·Brain, Behavior, and Immunity·Aravinthan Varatharaj, Ian Galea
Nov 13, 2010·Annals of the New York Academy of Sciences·Bengt F Belgardt, Jens C Brüning
Jun 20, 2006·Physiology & Behavior·Yi Zhang, Philip J Scarpace
Nov 20, 2009·Brain, Behavior, and Immunity·Véronique LafranceGiamal N Luheshi
Nov 3, 2009·Biochimica Et Biophysica Acta·William A Banks
Aug 31, 2014·Brain, Behavior, and Immunity·W A Banks
Dec 4, 2014·Metabolism: Clinical and Experimental·Stergios A Polyzos, Christos S Mantzoros
Sep 21, 2018·The Australian and New Zealand Journal of Psychiatry·Gerwyn MorrisMichael Berk
May 28, 2019·Nature Reviews. Endocrinology·William A Banks
Jul 7, 2018·Pflügers Archiv : European journal of physiology·Franziska SchusterWalter Raasch
May 1, 2020·Frontiers in Pharmacology·Olivia PatsalosHubertus Himmerich
Apr 15, 2021·Nutrition & Metabolism·Huimin LiuGuoqing Yang
Mar 28, 2007·Biochemical and Biophysical Research Communications·Hyun-Seuk MoonChong-Su Cho

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