Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in quality control in the Escherichia coli periplasm.

The Journal of Biological Chemistry
Hidenori MiyadaiHajime Tokuda

Abstract

Recent biochemical examination has revealed the presence of at least 90 different lipoproteins in Escherichia coli. Among previously identified lipoproteins, only an outer membrane lipoprotein, NlpE, is known to induce expression of the degP gene upon its overproduction. The degP gene encodes a periplasmic protease, which is thought to be involved in the digestion of unfolded proteins, and is essential for growth at high temperatures. However, it is not completely clear why NlpE overproduction causes degP expression. Moreover, among newly confirmed lipoproteins, there may be others that also induce degP expression. Therefore, we overproduced each of the 90 lipoproteins and examined the level of degP expression as beta-galactosidase activity by using a degP promoter-lacZ fusion. The extent of degP expression caused by NlpE overproduction was dependent on the mode of degP-lacZ fusion. On the other hand, new inner membrane lipoprotein YafY strongly induced degP expression irrespective of the mode of fusion even though the level of overproduced YafY was lower than that of NlpE. The induction of degP expression by YafY overproduction was dependent on the Cpx two-component system. Alteration of the lipoprotein-sorting signals of NlpE...Continue Reading

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Citations

May 23, 2006·Nature Reviews. Microbiology·Gary RowleyMark Roberts
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Suguru Okuda, Hajime Tokuda
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Thomas J SilhavySuzanne Walker
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Jul 5, 2017·Journal of Bacteriology·Géraldine Laloux, Jean-François Collet
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