Abstract
Cryptorchidism or local testicular heat treatment induces reversible oligospermia or azoospermia in rodents and humans via increased germ cell apoptosis. Research in this field has concentrated on the impact of heat on spermatogenesis, with rather little attention paid to the molecular effects of heat treatment on Leydig cell function. In the present study, we investigated the effects of exposure to heat stress on the proliferative activity and testosterone biosynthesis of Leydig cells. We subjected adult rats to a single local testicular heat treatment of water at 43°C for 30min. The expression of Leydig cell-specific markers, such as cholesterol side-chain cleavage (P450SCC) and 3?-hydroxysteroid dehydrogenase, was evaluated by immunohistochemistry and western blot analysis. The proliferative activity of Leydig cells was detected by immunostaining with proliferation-associated markers, including Ki67, bromodeoxyuridine and phosphohistone-H3 (pHH3). The mRNA and protein levels of cell cycle proteins and testosterone synthesis-related enzymes were measured by real-time polymerase chain reaction and western blot analysis, respectively. The testes of heat-treated rats contained 50% more Leydig cells than those of control rats, in...Continue Reading
References
May 1, 1980·Endocrinology·A H PayneK L Wong
Aug 1, 1995·International Journal of Andrology·R Mieusset, L Bujan
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·L BentonM P Hardy
Jan 1, 1997·Reproductive Toxicology·E D CleggG P Daston
Dec 24, 1997·Molecular and Cellular Endocrinology·B J Clark, D M Stocco
Mar 31, 1999·Endocrinology·Y H LueC Wang
Jun 16, 2000·Molecular and Cellular Endocrinology·V SriramanA J Rao
May 18, 2001·Endocrinology·X LiM Poutanen
Aug 22, 2001·Biology of Reproduction·S M Mendis-Handagama, H B Ariyaratne
Sep 22, 2001·Biology of Reproduction·B T AkingbemiM P Hardy
Sep 10, 2003·Endocrinology·Susana B RulliIlpo Huhtaniemi
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Benson T AkingbemiMatthew P Hardy
Dec 8, 2004·Endocrine Reviews·Anita H Payne, Dale B Hales
May 4, 2005·Journal of Andrology·Ben A WeissmanMatthew P Hardy
Dec 13, 2005·Endocrinology·Xue-Sen ZhangYi-Xun Liu
Aug 26, 2006·Cell Cycle·Stephanie LaufsHeike Allgayer
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Huizhen WangDaishu Han
Jun 7, 2008·Endocrinology·Min ChenYi-Xun Liu
Oct 7, 2011·Journal of Lipid Research·Walter L Miller, Himangshu S Bose
Sep 13, 2012·Toxicologic Pathology·Shin WakuiHitoshi Endou
Jun 14, 2013·Biology of Reproduction·Xi-Xia LiYi-Xun Liu
Sep 24, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Mathis GrossmannJeffrey D Zajac
Citations
Dec 29, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Zhihang YuanJing Wu
Apr 6, 2016·Theriogenology·Maíra Bianchi Rodrigues AlvesEneiva Carla Carvalho Celeghini
Apr 24, 2018·Molecular Reproduction and Development·Gamal BadrMohamed H Mahmoud
Aug 5, 2017·Systems Biology in Reproductive Medicine·Zhenlong KangYing Li
Jun 3, 2017·Endocrinology·Shintaro OkaHideyasu Matsuyama
Jul 24, 2020·Human & Experimental Toxicology·A DelkhoshF Abbasgholizadeh
Aug 3, 2017·Scientific Reports·Yuanlong HouJuan Su
Feb 16, 2019·Molecular Reproduction and Development·Raffaele Boni
Feb 28, 2021·Molecular and Cellular Endocrinology·Rashid A Aldahhan, Peter G Stanton
Mar 20, 2021·Biological Trace Element Research·Yongjie XiongShaojun He
Oct 21, 2020·Journal of Thermal Biology·Mustafa S AttaAli H El-Far
Oct 20, 2017·Theriogenology·Somayeh ShadmehrAlireza Amiri
Mar 29, 2019·Theriogenology·Augusto Bicalho Cruz VieiraElizete Rizzo
Aug 21, 2021·Andrologia·Fatma Bedia KarakayaSerap Sirvanci
Sep 9, 2021·Andrologia·Malgorzata Kotula-BalakJan Karol Wolski