Effects of long-term conduction block on membrane properties of reinnervated and normally innervated rat skeletal muscle

The Journal of Physiology
E PasinoA Cangiano

Abstract

1. Do motoneurons regulate muscle extrajunctional membrane properties through chemical (trophic) factors in addition to evoked activity? We addressed this question by comparing the effects of denervation and nerve conduction block by tetrodotoxin (TTX) on extrajunctional acetylcholine (ACh) sensitivity and action potential resistance to TTX in adult rats. 2. We applied TTX to sciatic or tibial nerves for up to 5 weeks using an improved blocking technique which completely suppresses conduction but avoids nerve damage. 3. Reinnervation by TTX-blocked axons had no effect on the high ACh sensitivity and TTX resistance induced by nerve crush. 4. Long-lasting block of intact nerves (up to 38 days) induced extrajunctional changes as pronounced as after denervation. At shorter times (3 days), however, denervation induced much larger changes than TTX block; such a difference is thus only transiently present in muscle. 5. The effects of long-lasting block were dose dependent. Dose levels (6.6 micrograms day-1) corresponding to those used in the literature to block the rat sciatic nerve induced muscle effects much smaller than those induced by denervation, confirming published data. Our novel finding is that equal effects are obtained usi...Continue Reading

Citations

Aug 10, 2006·European Journal of Applied Physiology·Menotti Midrio
Aug 15, 2015·Annual Review of Physiology·Terje Lømo
Aug 26, 1998·The Journal of Physiology·A WindischT Lømo
Jan 22, 2005·Journal of Neuroscience Methods·Vladimir N Martinov, Arild Njå
May 13, 1998·Acta Physiologica Scandinavica·K Gundersen
Dec 2, 2006·Journal of Neuroscience Research·Morgana FaveroAlberto Cangiano
Oct 1, 2005·Brain : a Journal of Neurology·S PrasarnpunJ B Harris
May 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Morgana FaveroAlberto Cangiano
Jun 12, 2013·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Morgana FaveroGiuseppe Busetto

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