Effects of lopap on human endothelial cells and platelets

Haemostasis
Ana Marisa Chudzinski-TavassiM A Lazzari

Abstract

Severe consumption coagulopathy has been detected in rats after Lopap (a prothrombin activator from Lonomia obliqua caterpillar bristles) infusion and in humans after accidental contact with L. obliqua bristles. However, platelet count and antithrombin (AT) levels were only modestly affected, suggesting that a different form of blood coagulation activation may be involved in this hemorrhagic syndrome. Here we describe that Lopap had no effect on aggregation of washed human platelets induced by several agonists, suggesting that it might not impair platelet function in vivo. AT was able to inhibit the amidolytic activity of thrombin generated by incubation of Lopap with prothrombin in a purified system, which may be different from that generated by the prothrombinase complex in vivo. The surface expression of both ICAM-1 and E-selectin but not of VCAM-1 was upregulated by Lopap in cultured HUVEC, suggesting that it may behave differently from other mediators, such as thrombin and tumor necrosis factor-alpha.

Citations

Jun 21, 2005·Biochemical and Biophysical Research Communications·Márcio FritzenAna Marisa Chudzinski-Tavassi
May 19, 2006·Pathophysiology of Haemostasis and Thrombosis·Ana Marisa Chudzinski-Tavassi, Miryam Paola Alvarez Flores
Jan 22, 2005·Microscopy Research and Technique·Gustavo Henrique Da SilvaMaria Alice Da Cruz-Höfling
Aug 9, 2011·Toxicon : Official Journal of the International Society on Toxinology·Belsy GuerreroSara Lucena
Aug 13, 2009·Toxicon : Official Journal of the International Society on Toxinology·Kaline WaismamSandra H P Farsky
Mar 18, 2008·Toxicon : Official Journal of the International Society on Toxinology·Maria Esther Ricci-SilvaAna Marisa Chudzinski-Tavassi
Feb 27, 2007·Toxicon : Official Journal of the International Society on Toxinology·Linda Christian Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Gustavo Henrique da SilvaMaria Alice da Cruz-Höfling
Jul 31, 2004·Toxicon : Official Journal of the International Society on Toxinology·Jairo José Caovilla, Elvino José Guardão Barros
Apr 7, 2006·Biochemical and Biophysical Research Communications·Miryam Paola Alvarez FloresAna Marisa Chudzinski-Tavassi
Nov 29, 2005·Toxicon : Official Journal of the International Society on Toxinology·Geni Portela GamborgiElvino J G Barros
Jul 11, 2012·Toxicon : Official Journal of the International Society on Toxinology·Vany Nascimento-SilvaChristina Barja-Fidalgo

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