Effects of low AIPL1 expression on phototransduction in rods.

Investigative Ophthalmology & Visual Science
Clint L MakinoA M Dizhoor

Abstract

To investigate the impact of aryl hydrocarbon receptor-interacting protein-like (AIPL)-1 on photoreception in rods. Photoresponses of mouse rods expressing lowered amounts of AIPL1 were studied by single-cell and electroretinogram (ERG) recordings. Phototransduction protein levels and enzymatic activities were determined in biochemical assays. Ca2+ dynamics were probed with a fluorescent dye. Comparisons were made to rods expressing mutant Y99C guanylate cyclase activating protein (GCAP)-1, to understand which effects arose from elevated dark levels of cGMP and Ca2+. Except for PDE, transduction protein levels were normal in low-AIPL1 retinas, as were guanylate cyclase (GC), rhodopsin kinase (RK), and normalized phosphodiesterase (PDE) activities. Y99C and low-AIPL1 rods were more sensitive to flashes than normal, but flash responses of low-AIPL1 rods showed an abnormal delay, reduced rate of increase, and longer recovery not present in Y99C rod responses. In addition, low-AIPL1 rods but not Y99C rods failed to reach the normal light-induced minimum in Ca2+ concentration. Reduced AIPL1 delayed the photoresponse, decreased its amplification constant, slowed a rate-limiting step in its recovery, and limited the light-induced decr...Continue Reading

Citations

Dec 1, 2012·Journal of Ocular Biology, Diseases, and Informatics·S MuthukumaranMeena Revathi Valliappan
May 25, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elena V OlshevskayaAlexander M Dizhoor
Aug 19, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael L WoodruffAlexander M Dizhoor
Feb 3, 2009·Biophysical Journal·Xiao-Hong WenClint L Makino
Oct 3, 2019·Ophthalmic Genetics·Ling WanLulin Huang

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