PMID: 9181608Dec 1, 1996Paper

Effects of low concentrations of cis- and trans-urocanic acid on cytokine elaboration by keratinocytes

Photodermatology, Photoimmunology & Photomedicine
P RedondoE Quintanilla

Abstract

The urocanic acid cis isomer (cis-UCA) is a possible cutaneous photoreceptor for the immunomodulatory phenomena that follow ultraviolet B irradiation. Several experiments in animals show an inhibitory action of cis-UCA on cellular immunity. However, the action of cis-UCA on the synthesis of cytokines in keratinocytes remains unknown. Long-term cultures of normal human keratiocytes were prepared in a serum-free medium, and stimulated with 1 microgram/ml of phorbol 12-myristate 13-acetate (TPA) and UCA or UVB-UCA (10-100 micrograms/ml). Synthesis of the following cytokines was measured using ELISA and Northern blot techniques: TNF-alpha, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TGF-beta 1. TPA increased TNF-alpha protein levels in culture supernatants. No changes in Il-1 alpha and IL-1 beta protein levels were detected in basal culture supernatant after TPA stimulus. TPA augmented RNA expression for TNF-alpha, IL-1 alpha, IL-1 beta and TGF-beta 1. UCA isomers did not induce cytokine changes in protein synthesis. Expression of IL-1 alpha and IL-1 beta genes was increased after exposure to 100 micrograms/ml UVB-UCA (70 micrograms/ml cis-UCA). A slight increase in TNF-alpha RNA expression was detected when the dose of UVB-UCA reached 1...Continue Reading

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Citations

Jun 3, 1999·The British Journal of Dermatology·M S DuthieM Norval
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Susan L Walker, Antony R Young
Jun 26, 1999·The Journal of Investigative Dermatology·V E ReeveN Nishimura
Dec 27, 2008·The Journal of Investigative Dermatology·Vivienne E ReeveLynette Blyth
Nov 18, 2003·Photodermatology, Photoimmunology & Photomedicine·M R PraterS D Holladay
Apr 3, 2001·Photochemistry and Photobiology·M Zak-PrelichR C McKenzie
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuyo KanekoSusan L Walker
Mar 28, 2002·Journal of the American Chemical Society·Nicole Haralampus-GrynaviskiJohn D Simon

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