Effects of Low Dissolved-Oxygen Concentrations on Poly-(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by Alcaligenes eutrophus.

Applied and Environmental Microbiology
G LefebvreG Braunegg

Abstract

The bacterial copolyester poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) was produced with Alcaligenes eutrophus DSM 545 from glucose and sodium propionate in a fed-batch fermentation with both nitrogen limitation and low dissolved-oxygen concentrations. When the dissolved-oxygen content was kept between 1 and 4% of air saturation during the polymer accumulation phase, the yield of 3-hydroxybutyrate (3HB) monomer from glucose was not affected, but the propionate-to-3-hydroxyvalerate (3HV) monomer yield was two to three times (0.48 to 0.73 mol of 3HV mol of propionate consumed(sup-1)) that observed in a control experiment (0.25 mol mol(sup-1)), where the accumulation-phase dissolved-oxygen concentration was 50 to 70% of air saturation. The overall polymer productivity of the fermentation was somewhat decreased by low dissolved-oxygen contents, owing to a slower 3HB production rate. The effect of a low dissolved-oxygen concentration is probably attributable to a reduction of the oxygen-requiring decarbonylation of propionyl-coenzyme A (CoA) to acetyl-CoA.

References

Oct 1, 1976·Applied and Environmental Microbiology·R Repaske, A C Repaske
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·W D Luzier
Jul 1, 1990·Applied and Environmental Microbiology·B A RamsayJ A Ramsay
Jan 1, 1994·Journal of Chemical Technology and Biotechnology·J D SheppardS F Barrington
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C NawrathC Somerville
Jul 1, 1996·Applied and Environmental Microbiology·M D WilliamsD H Sherman
Oct 1, 1990·Applied and Environmental Microbiology·J L BertrandC Chavarie

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Citations

Feb 5, 2021·Bioresource Technology·Stanislav ObrucaMartin Koller
Jul 5, 2018·World Journal of Microbiology & Biotechnology·Warren BluntNazim Cicek
Feb 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin Koller
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman

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