Effects of low-intensity focused ultrasound on the mouse submandibular gland

Ultrasound in Medicine & Biology
Jun ShutoMasashi Suzuki

Abstract

Ultrasound is expected to make a considerable contribution to drug delivery systems (DDSs). We tested the hypothesis that low-intensity focused ultrasound (LIFU) increases vessel permeability in the mouse submandibular gland without causing parenchymal damage. In a preliminary study, LIFU at 3 W/cm2 with a 50% duty cycle for 2 minutes did not cause histologic damage. We therefore applied LIFU to mouse submandibular gland at these conditions before and after injecting horseradish peroxidase. Single labeling laser scanning confocal microscopy revealed positive horseradish peroxidase staining around the excretory ducts in the mucous-producing part of the gland, but absence of staining in control glands. Immunostaining for fibrinogen was positive in the same region. Fibrinogen is an intravascular protein that does not pass through intact vessels. These findings suggest that LIFU increases vessel permeability and disruption without destruction. It is anticipated that this process will be useful in establishing a DDS that uses LIFU.

References

Jan 1, 1992·Ultrasound in Medicine & Biology·A H Saad, G M Hahn
Oct 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J C Adams
May 1, 1992·Journal of Vascular and Interventional Radiology : JVIR·K Tachibana
Jun 1, 1991·International Journal of Radiation Biology·G H HarrisonH A Eddy
Jul 1, 1990·The British Journal of Radiology·P LoverockP R Imrie
Mar 1, 1989·Japanese Journal of Cancer Research : Gann·N YumitaS Umemura
Jan 1, 1989·Ultrasound in Medicine & Biology·M A DinnoJ Wu
Jan 1, 1995·Ultrasound in Medicine & Biology·C W FrancisC Cox
Feb 1, 1997·Lancet·K TachibanaE Morioka
Oct 23, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M HippiusA Hoffmann
May 5, 1999·Lancet·K TachibanaK Tamura
Mar 13, 2002·Circulation·Yoshiaki TaniyamaRyuichi Morishita
Apr 30, 2002·Ultrasound in Medicine & Biology·Ali H MesiwalaPierre D Mourad
Oct 29, 2002·Ultrasound in Medicine & Biology·Ludwig J Weimann, Junru Wu
Dec 7, 2002·Ultrasound in Medicine & Biology·Ana Rosa Crisci, Affonso Luiz Ferreira
Jul 10, 2003·Lancet Neurology·Michael Daffertshofer, Michael Hennerici
Nov 5, 2003·Genesis : the Journal of Genetics and Development·Sho OhtaGen Yamada
May 4, 2004·Ultrasound in Medicine & Biology·Cheri X DengJianmin Cui
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Atsushi HashizumeYohki Hieda
Oct 9, 2004·Biochimica Et Biophysica Acta·Shao-Ling Huang, Robert C MacDonald
Nov 30, 2004·Biomolecular Engineering·Shan-hui Hsu, Tsung-bin Huang
Mar 8, 2005·Ultrasound in Medicine & Biology·Rong-Sen YangWin-Li Lin

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Citations

Jan 15, 2013·Ultrasound in Medicine & Biology·Kanako NodaMasashi Suzuki

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