Effects of Low Phytanic Acid-Concentrated DHA on Activated Microglial Cells: Comparison with a Standard Phytanic Acid-Concentrated DHA

Neuromolecular Medicine
María Belén Ruiz-RosoBeatriz Martín-Fernández

Abstract

Docosahexaenoic acid (DHA, 22:6 n-3) is an essential omega-3 (ω-3) long chain polyunsaturated fatty acid of neuronal membranes involved in normal growth, development, and function. DHA has been proposed to reduce deleterious effects in neurodegenerative processes. Even though, some inconsistencies in findings from clinical and pre-clinical studies with DHA could be attributed to the presence of phytanic acid (PhA) in standard DHA treatments. Thus, the aim of our study was to analyze and compare the effects of a low PhA-concentrated DHA with a standard PhA-concentrated DHA under different neurotoxic conditions in BV-2 activated microglial cells. To this end, mouse microglial BV-2 cells were stimulated with either lipopolysaccharide (LPS) or hydrogen peroxide (H2O2) and co-incubated with DHA 50 ppm of PhA (DHA (PhA:50)) or DHA 500 ppm of PhA (DHA (PhA:500)). Cell viability, superoxide anion (O2-) production, Interleukin 6 (L-6), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), glutathione peroxidase (GtPx), glutathione reductase (GtRd), Caspase-3, and the brain-derived neurotrophic factor (BDNF) protein expression were explored. Low PhA-concentrated DHA protected against LPS or H2O2-induced cell viability reducti...Continue Reading

References

Jan 1, 1995·Progress in Lipid Research·A J McColl, C A Converse
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Krishnan SriramJames P O'Callaghan
Jan 25, 2003·Free Radical Biology & Medicine·Victoria C Stewart, Simon J R Heales
Aug 19, 2003·The Journal of Biological Chemistry·Victor L MarcheselliNicolas G Bazan
Sep 30, 2004·Journal of Neuropathology and Experimental Neurology·Luisa Minghetti
Nov 24, 2004·Progress in Retinal and Eye Research·John Paul SanGiovanni, Emily Y Chew
Jan 3, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Georg ReiserStefan Kahlert
Jan 18, 2006·The Journal of Biological Chemistry·Peter Schönfeld, Georg Reiser
Jan 31, 2006·The Proceedings of the Nutrition Society·Christine M Williams, Graham Burdge
Feb 10, 2006·Science's STKE : Signal Transduction Knowledge Environment·John A Glomset
Apr 18, 2006·Experimental Gerontology·Peter SchönfeldGeorg Reiser
Jun 30, 2006·Journal of Neurochemistry·Salvador Moncada, Juan P Bolaños
Jul 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Oleg ButovskyMichal Schwartz
Apr 26, 2007·The American Journal of Pathology·Leah T RemingtonTrevor Owens
Aug 30, 2007·Cancer Causes & Control : CCC·Peter WallströmElisabet Wirfält
Jun 17, 2008·Free Radical Biology & Medicine·Doris L Lefkowitz, Stanley S Lefkowitz
Oct 3, 2008·The Journal of Nutritional Biochemistry·Li-he JiangZhi-rong Yang
Oct 31, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lauriane UlmannFrancois Rassendren
Jun 16, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Greg M ColeSally A Frautschy
Aug 18, 2009·Journal of Neurochemistry·Dehua CaoHee-Yong Kim
Oct 22, 2009·Neuromolecular Medicine·Eitan OkunThiruma V Arumugam
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Hee-Yong KimYang-Suk Kim
Mar 23, 2010·Cell·Charles A Dinarello
Dec 9, 2010·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Leo Galland
Apr 23, 2011·Journal of Neuroinflammation·Jie WenYumin Zhang
Jun 21, 2011·Biochimica Et Biophysica Acta·Ronald J A WandersSacha Ferdinandusse
Jan 27, 2012·The Journal of Nutrition·Philip C Calder
Mar 7, 2012·Biotechnology Letters·Wei-Chun TuRobert A Gibson

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Citations

Aug 26, 2020·International Journal of Molecular Sciences·Paola BozzatelloSilvio Bellino

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