Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids

Stem Cell Research & Therapy
Esmeralda Magro-LopezAlberto Zambrano

Abstract

Organoids from human pluripotent stem cells are becoming suitable models for studies of organ development, drug screening, regenerative medicine, and disease modeling. Three-dimensional minilungs in Matrigel culture have recently been generated from human embryonic stem cells. These particular organoids, named lung bud organoids, showed branching airway and early alveolar structures resembling those present in lungs from the second trimester of human gestation. We show here that the treatment of such organoids with a lung and airway epithelial maturation cocktail containing dexamethasone drives lung bud organoids to the formation of paddle-racquet like structures. This strategy may help to increase the versatility of lung organoids and to generate structures more advanced than the original branching texture.

References

Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linda W GonzalesPhilip L Ballard
Oct 15, 2009·Annual Review of Medicine·Jeffrey A WhitsettTimothy E Weaver
Apr 10, 2012·Cell Stem Cell·Tyler A LongmireDarrell N Kotton
Dec 3, 2013·Nature Biotechnology·Sarah X L HuangHans-Willem Snoeck
Jan 8, 2014·The Journal of Cell Biology·Alberto ZambranoAna Aranda
Feb 6, 2015·Nature Protocols·Sarah X L HuangHans-Willem Snoeck
Apr 25, 2017·Nature Cell Biology·Ya-Wen ChenHans-Willem Snoeck
Dec 14, 2017·Viruses·Esmeralda Magro-LopezAlberto Zambrano

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Methods Mentioned

BETA
fluorescence microscopy
PCR

Software Mentioned

AxioVision

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