Abstract
Metastasis-associated in colon cancer 1 (MACC1) has been shown to play a critical role in several types of cancer. The purposes of this study were to evaluate MACC1 expression in cervical cancer and determine role of MACC1 small interference RNA (siRNA) in the growth and progression of cervical cancer. Immunohistochemical analysis of MACC1 expression was performed in different cervical lesion tissues. siRNA knockdown of MACC1 was performed. Cytoskeletal staining, RT-PCR, Western blot technology, transwell migration, MTT, and flow cytometry were used for identification of the functional roles of MACC1 siRNA in HeLa cells. Immunohistochemistry demonstrated that MACC1 overexpression was detected in cervical cancer tissues. MACC1 siRNA transfection remarkably affected HeLa cell biological behaviors. Expression of MACC1 in HeLa cells was significantly down-regulated by MACC1 siRNA. In addition, knockdown of MACC1 in HeLa cells caused a significant decrease in cell proliferation or migration, and increased cell apoptosis rate. Flow cytometry showed that MACC1 siRNA may inhibit cell proliferation by interfering with cell mitosis and cell cycle progression. These results suggest that MACC1 is a novel biomarker for cervical cancer diagn...Continue Reading
References
Feb 15, 1991·Science·D P BottaroS A Aaronson
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jenn-Yah YuDavid L Turner
Jan 15, 2003·Clinical Microbiology Reviews·Eileen M Burd
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Carmen BirchmeierGeorge F Vande Woude
Jan 1, 2004·Nature Genetics·Toshio OtaSumio Sugano
May 5, 2005·Molecular and Cellular Biology·M Cristina StellaPaolo M Comoglio
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luca Toschi, Pasi A Jänne
Dec 23, 2008·Nature Medicine·Ulrike SteinPeter M Schlag
Apr 4, 2009·Genome Medicine·Lisa A Boardman
Apr 11, 2009·Gynecologic Oncology·Jinke LiLina Hu
Jun 27, 2009·Cell Cycle·Ulrike SteinFranziska Arlt
Aug 12, 2009·The International Journal of Biochemistry & Cell Biology·Franziska Arlt, Ulrike Stein
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Aug 8, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·George R BlumenscheinAna M Gonzalez-Angulo
Dec 13, 2012·International Journal of Oncology·Atsushi MatsumuraEigo Otsuji
Jul 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Holger CramerAnna Paul
Citations
Jun 6, 2015·Molecular Medicine Reports·Longfeng SunAiping Wang
Dec 2, 2014·Oncology Reports·Peiwu LiZhongxue Fu
Feb 27, 2016·Scientific Reports·Jorge BarbazanPradipta Ghosh
Jun 20, 2018·Molecular Medicine Reports·Shumei WangXiaoling Ji
Oct 12, 2019·Experimental and Therapeutic Medicine·Fanxu MengBaosheng Sun
Jun 7, 2020·Cell Communication and Signaling : CCS·Tim HohmannFaramarz Dehghani
Jan 14, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Clara LemosUlrike Stein
Feb 20, 2021·Cancer Management and Research·Hongwei ChenLimei Zhao