Effects of macromolecular crowding on glycoprotein processing enzymes

Journal of the American Chemical Society
Kiichiro TotaniYukishige Ito

Abstract

Intracellular environments are highly crowded due to the presence of various biomacromolecules. In this study, we estimated the property of the endoplasmic reticulum glucosidase II (G-II) under macromolecular crowding conditions. A crowded milieu that contains bovine serum albumin greatly enhanced the second trimming step (cleavage 2), which deglucosylates Glc1Man9GlcNAc2, but not the first trimming step (cleavage 1), which removes the terminal glucose residue from Glc2Man9GlcNAc2. A similar effect was obtained with ribonuclease A and high molecular weight polyethylene glycol 20,000. An analysis of CD spectra suggested that G-II enhanced its cleavage 2 activity through conformational change. We also investigated the effects of molecular crowding on other N-linked glycan-processing enzymes, UDP-Glc:glycoprotein glucosyltransferase and 1,2-alpha-mannosidase. Our results indicate that the kinetics of glycan processing under crowded conditions may be quite different from those measured in dilute buffers.

References

Nov 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J J Aragón, A Sols
Aug 29, 2001·Biochemistry·E S TrombettaA Helenius
Oct 9, 2001·Trends in Biochemical Sciences·V Niggli
Feb 14, 2002·Trends in Biochemical Sciences·Alan S Verkman
Sep 5, 2003·Nature·Vlatko Vedral
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Beatriz FarruggiaGuillermo Picó
Jun 11, 2004·Annual Review of Biochemistry·Ari Helenius, Markus Aebi
Jan 4, 2005·Biochemistry. Biokhimii︠a︡·N A ChebotarevaN B Livanova
May 2, 2006·Bioorganic & Medicinal Chemistry·Kiichiro TotaniYukishige Ito
Jul 11, 2006·Journal of Cell Science·Allen P Minton
Aug 31, 2006·The Journal of Biological Chemistry·Kiichiro TotaniYukishige Ito

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Citations

Oct 8, 2013·International Journal of Biological Macromolecules·Manish Kumar SutharJitendra Kumar Saxena
Dec 31, 2008·Biochemistry·Gary J PielakImola G Zigoneanu
Jan 15, 2009·Journal of the American Chemical Society·Conggang Li, Gary J Pielak
Jul 17, 2009·Molecular Biology of the Cell·Ivan D StiglianoCecilia D'Alessio
Apr 8, 2011·Molecular Biology of the Cell·Ivan D StiglianoCecilia D'Alessio
Feb 9, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yukishige Ito, Yoichi Takeda
Dec 10, 2013·Bioscience, Biotechnology, and Biochemistry·Yukishige Ito, Yoichi Takeda
Feb 13, 2014·Journal of Biochemistry·Jun-Ichi AikawaYukishige Ito
Dec 17, 2014·International Journal of Molecular Sciences·Irina M KuznetsovaVladimir N Uversky
Aug 1, 2015·FEBS Letters·Julio J Caramelo, Armando J Parodi
Jan 5, 2010·Seminars in Cell & Developmental Biology·Cecilia D'AlessioArmando J Parodi
Nov 26, 2009·Biochimica Et Biophysica Acta·Irina Pozdnyakova, Pernilla Wittung-Stafshede
Sep 19, 2015·Journal of the American Chemical Society·Joost GroenWilhelm T S Huck
May 31, 2015·Bioprocess and Biosystems Engineering·Yuichi NakagawaKazuya Koumoto
Mar 20, 2013·Biochimica Et Biophysica Acta·Tali GidalevitzYair Argon
Aug 5, 2009·Biophysical Journal·Yael PhillipGideon Schreiber
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alessandro T CaputoNicole Zitzmann
Oct 26, 2016·Protein Science : a Publication of the Protein Society·Tadashi SatohKoichi Kato
May 5, 2017·Bioscience, Biotechnology, and Biochemistry·Masayuki OkuyamaAtsuo Kimura
Apr 4, 2017·Chembiochem : a European Journal of Chemical Biology·Taiki KuribaraKiichiro Totani
Oct 4, 2017·Protein Science : a Publication of the Protein Society·Luis C AcostaAnnelise H Gorensek-Benitez
Jun 2, 2016·Organic & Biomolecular Chemistry·Masayuki IzumiYasuhiro Kajihara
Jan 15, 2015·Chemistry : a European Journal·Kohki FujikawaYukishige Ito
Oct 24, 2015·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Kohki FujikawaYukishige Ito
Jun 30, 2010·Organic & Biomolecular Chemistry·Akihiro IshiwataYukishige Ito
Jul 17, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jiahui WangZhimin He
Mar 19, 2013·Chembiochem : a European Journal of Chemical Biology·Hikaru MatsushimaKiichiro Totani
Oct 28, 2020·Chemistry : a European Journal·Yukishige ItoYoichi Takeda
Nov 2, 2019·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Temidayo OgunmoyoleAdeyinka Afolayan
Jan 10, 2020·The Journal of Physical Chemistry. B·Marin MatićFrancesco Piazza
Aug 19, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zahoor Ahmad ParrayAsimul Islam
Dec 19, 2009·The Journal of Physical Chemistry. B·Hongwei XiaGuangzhao Zhang
Sep 23, 2008·Biochemistry·Eran BosisMenachem Gutman
May 21, 2021·Journal of Molecular Recognition : JMR·Samra Hasan, Aabgeena Naeem

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