Effects of maternal antenatal glucocorticoid treatment on apoptosis in the ovine fetal cerebral cortex.

Journal of Neuroscience Research
Shadi N MalaebBarbara S Stonestreet

Abstract

We examined the effects of single and multiple maternal glucocorticoid courses on apoptosis in the cerebral cortices of ovine fetuses (CC). Ewes received single dexamethasone or placebo courses at 104-106 or 133-135 days or multiple courses between 76-78 and 104-106 days gestation. In the single-course groups, ewes received four 6 mg dexamethasone or placebo injections every 12 hr for 48 hr. Multiple-course groups received the same treatment once per week for 5 weeks. Neuronal and nonneuronal apoptotic cell numbers per square millimeter were determined with TUNEL and NeuN staining and with caspase-3 enzyme activity on CC tissues harvested at 106-108 (70%) or 135-137 (90%) days of gestation. Apoptotic cell numbers and caspase-3 activity were 50% lower (P < 0.02) after single placebo courses at 90% than 70% gestation; 90% of apoptotic cells were (P < 0.01) nonneuronal at both ages. Nonneuronal apoptotic cells and caspase-3 activity were 40% and 20% lower (P < 0.02) after single dexamethasone than placebo courses at 70%, but not 90%, gestation. Caspase-3 activity was 20% lower (P < 0.01) after multiple dexamethasone than placebo courses, but apoptotic cell number did not differ. We conclude that nonneuronal apoptosis represents th...Continue Reading

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Citations

Jul 10, 2010·Stroke; a Journal of Cerebral Circulation·Govindaiah VinukondaPraveen Ballabh
Mar 4, 2014·European Journal of Pharmacology·Sofia F MakrydimaDemetrios P Kassanos
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Jun 25, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cindy LeeVirender K Rehan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis