Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro

Pharmaceutical Biology
Xue-Gong QinYu-Dong Cui

Abstract

Matrine, one of the main active components extracted from dry roots of Sophora flavescens Ait (Leguminosae), has been reported to have anticancer effects on a number of cancer cell lines, but the anticancer mechanism of matrine remains elusive. This study shows that matrine also displays anticancer activity on human hepatocellular carcinoma (HepG2) cells. In this work, the optimal cultivation condition for HepG2 cells was determined using the combinatorial orthogonal test design [L18 (21 x 37)]. Exposure of HepG2 cells to matrine resulted in inhibition of proliferation in both a time- and dose-dependent manner, as measured by morphology observation, hematoxylin and eosin (H&E) staining, and MTT assay (p<0.05). Further immunohistochemical analyses revealed that the expression of alpha fetal protein (AFP), proliferating cell nuclear antigen (PCNA), C-myc and Bcl-2 was down-regulated significantly, but the expression of Bax was up-regulated higher than untreated cells. The results demonstrated that matrine inhibited HepG2 cells proliferation primarily via up-regulating or down-regulating expression of the tumor relevant proteins.

References

Aug 10, 2000·Nature Cell Biology·M SaitoP H Schlesinger
Aug 8, 2001·Leukemia Research·L P ZhangY J He
Jan 31, 2002·World Journal of Gastroenterology : WJG·Li ZhangXiao-Ning Wang
Aug 29, 2003·The American Journal of Chinese Medicine·Jianping LiuMin Yang
Dec 4, 2003·Current Opinion in Cell Biology·Tomomi Kuwana, Donald D Newmeyer
Oct 18, 2005·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·P Merle
Oct 18, 2005·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Hollins P ClarkRonald J Zagoria
Mar 9, 2007·European Journal of Pharmacology·Lijun ZhangLei Wei

❮ Previous
Next ❯

Citations

Aug 26, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Bowen ZhaoQian Liu
Nov 30, 2013·International Journal of Molecular Sciences·Li WangBushan Xie
Dec 27, 2015·Asian Pacific Journal of Tropical Medicine·Guan-Li ZhangLu Zhang
Mar 4, 2014·Chemico-biological Interactions·Siyan ChenNanhong Tang
Sep 9, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Homervergel G Ong, Young-Dong Kim
Feb 14, 2013·International Journal of Oncology·Yumin LiXiangting Zeng
Oct 26, 2018·European Journal of Medicinal Chemistry·Haroon Ur RashidJun Jiang
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jinrui WeiLichuan Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.