Abstract
Medetomidine, an antifouling candidate, was investigated for its effects on cytochrome P4501A (CYP1A) activity in fish. Rainbow trout (Oncorhynchus mykiss), turbot (Psetta maxima), and Atlantic cod (Gadus morhua) were exposed to medetomidine either via i.p. injection (<5 micromol (1mg)/kg) or via water (<50 nM). Enzyme activity was measured as ethoxyresorufin-O-deethylase (EROD) activity in liver microsomes. There was a small (2-7-fold) increase in EROD activity in rainbow trout. In turbot, EROD activity increased (4-fold) after injection, while a non-significant (50%) decrease was observed after water exposure. No effects on EROD activities were observed in Atlantic cod. In vitro inhibition studies of EROD activities in liver microsomes from all three species showed that medetomidine was a very potent CYP1A inhibitor. Thus, median inhibition values (IC(50)) were 35+/-10nM for rainbow trout, 47+/-17 nM for turbot, and 111+/-70 nM for Atlantic cod. These observed effects suggest that medetomidine interferes with CYP1A-dependent metabolism of xenobiotics in these fish species.
References
Dec 1, 1992·Anesthesiology·E D KharaschR Labroo
Sep 1, 1991·Pharmacology & Toxicology·O PelkonenR Lammintausta
Jan 1, 1989·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J Bach, J Snegaroff
Aug 1, 1994·Toxicology and Applied Pharmacology·M E Hahn, J J Stegeman
Jul 11, 1998·Biochimica Et Biophysica Acta·A YawetzJ J Stegeman
Jun 15, 1999·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·M B RooneyJ Wimsatt
Sep 28, 2000·The Science of the Total Environment·M A Champ
Jun 16, 2001·Aquatic Toxicology·A SturmH Segner
Jun 25, 2002·Chemosphere·Nikolaos VoulvoulisJohn N Lester
Jun 1, 2004·Aquatic Toxicology·Annemarie P van Wezel, P van Vlaardingen
Jun 8, 2004·Environmental Toxicology and Chemistry·Tove HegelundMalin C Celander
Aug 27, 2004·Journal of the American Veterinary Medical Association·Thomas D WilliamsBarbara A Block
Aug 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BabínJ M Navas
Dec 24, 2005·Biofouling·Juan BellasAke Granmo
Apr 4, 2006·Chemosphere·Juan BellasKe Granmo
Citations
May 19, 2012·Biofouling·Cecilia OhlausonHans Blanck
Sep 10, 2014·Environmental Toxicology and Pharmacology·Wen-Jun ZhuShu Li
Jun 12, 2010·Chemosphere·Anna LennquistLars Förlin
Feb 26, 2010·Marine Environmental Research·Anna LennquistLars Förlin
May 1, 2009·Marine Environmental Research·Annelie HilvarssonAke Granmo
Mar 9, 2011·Environmental Toxicology·Zhi-Hua LiTomas Randak
Mar 4, 2014·Ecotoxicology and Environmental Safety·Jianchao LiuZhenhua Yan
Jun 28, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Anna LennquistLars Förlin
Oct 26, 2010·Ecotoxicology and Environmental Safety·Zhi-Hua LiTomas Randak
Dec 7, 2013·Journal of Feline Medicine and Surgery·Thierry BethsKirby Pasloske
Apr 15, 2010·Biofouling·K V Thomas, S Brooks