Effects of membrane mimetics on cytochrome P450-cytochrome b5 interactions characterized by NMR spectroscopy.

The Journal of Biological Chemistry
Meng ZhangAyyalusamy Ramamoorthy

Abstract

Mammalian cytochrome P450 (P450) is a membrane-bound monooxygenase whose catalytic activities require two electrons to be sequentially delivered from its redox partners: cytochrome b5 (cytb5) and cytochrome P450 reductase, both of which are membrane proteins. Although P450 functional activities are known to be affected by lipids, experimental evidence to reveal the effect of membrane on P450-cytb5 interactions is still lacking. Here, we present evidence for the influence of phospholipid bilayers on complex formation between rabbit P450 2B4 (CYP2B4) and rabbit cytb5 at the atomic level, utilizing NMR techniques. General line broadening and modest chemical shift perturbations of cytb5 resonances characterize CYP2B4-cytb5 interactions on the intermediate time scale. More significant intensity attenuation and a more specific protein-protein binding interface are observed in bicelles as compared with lipid-free solution, highlighting the importance of the lipid bilayer in stabilizing stronger and more specific interactions between CYP2B4 and cytb5, which may lead to a more efficient electron transfer. Similar results observed for the interactions between CYP2B4 lacking the transmembrane domain (tr-CYP2B4) and cytb5 imply interaction...Continue Reading

References

Dec 29, 1975·Biochimica Et Biophysica Acta·J W Depierre, G Dallner
Jan 1, 1977·Annual Review of Biochemistry·J W DePierre, L Ernster
Jan 1, 1977·FEBS Letters·M Ingelman-Sundberg, H Glaumann
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·S KominamiS Takemori
Nov 15, 1990·Analytical Biochemistry·I H van StokkumF C Groen
Jun 1, 1985·Biochemical Pharmacology·E Canova-DavisL Waskell
Apr 3, 1973·Biochimica Et Biophysica Acta·R Coleman
Jan 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R W EstabrookJ Werringloer
Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Aug 15, 1993·Archives of Biochemistry and Biophysics·E M GillamF P Guengerich
Nov 4, 1998·Archives of Biochemistry and Biophysics·M L Shank-RetzlaffS G Sligar
Mar 10, 2001·Protein Expression and Purification·A S SaribasL Waskell
Jul 4, 2001·The Journal of Biological Chemistry·R M Garavito, S Ferguson-Miller
Jan 12, 2002·Current Opinion in Structural Biology·A Arora, L K Tamm
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy H Bayburt, Stephen G Sligar
Oct 22, 2002·Lancet·Daniel W Nebert, David W Russell
Dec 5, 2002·Archives of Biochemistry and Biophysics·David R Nelson
Mar 3, 2004·Molecular Interventions·F Peter Guengerich
Sep 24, 2004·Journal of Molecular Recognition : JMR·Miguel Prudêncio, Marcellus Ubbink
Feb 1, 2005·Archives of Biochemistry and Biophysics·Tsutomu ShimadaF Peter Guengerich
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Alexander N VolkovMarcellus Ubbink
Aug 30, 2005·Biochemical and Biophysical Research Communications·F Peter GuengerichCheryl J Bartleson
Apr 6, 2006·The AAPS Journal·F Peter Guengerich
Nov 4, 2006·Cell·Erin E MatthewsDonald M Engelman
May 10, 2007·Journal of the American Chemical Society·Ulrich H N DürrAyyalusamy Ramamoorthy
Dec 19, 2007·The Journal of Biological Chemistry·Haoming ZhangLucy Waskell
Sep 27, 2008·Journal of the American Chemical Society·Donghan LeeChristian Griesinger
Sep 23, 2009·Chemical Reviews·Paul R Ortiz de Montellano
Nov 5, 2010·Journal of Biomolecular NMR·Alexander N VolkovNico A J van Nuland
Nov 9, 2010·Archives of Biochemistry and Biophysics·Sang-Choul Im, Lucy Waskell

❮ Previous
Next ❯

Citations

Mar 16, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Megh Raj BhattLisandra L Martin
Sep 24, 2016·Quarterly Reviews of Biophysics·Mohammed KaplanMarc Baldus
Feb 9, 2017·Chemical Reviews·Ilia G Denisov, Stephen G Sligar
Apr 21, 2016·Chemical Science·Areetha D'SouzaSurajit Bhattacharjya
May 22, 2018·Chemical Communications : Chem Comm·Katherine A GentryAyyalusamy Ramamoorthy
Nov 17, 2017·Chemical Communications : Chem Comm·Thirupathi RavulaAyyalusamy Ramamoorthy
Aug 11, 2018·Chemical Communications : Chem Comm·Thirupathi RavulaAyyalusamy Ramamoorthy
Jul 12, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Carlo Barnaba, Ayyalusamy Ramamoorthy
Sep 12, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Chun-Zhi AiYi-Zhou Jiang
Dec 20, 2018·International Journal of Molecular Sciences·Diana CampeloMichel Kranendonk
Nov 10, 2020·Protein Science : a Publication of the Protein Society·Stephen G Sligar, Ilia G Denisov
Jan 19, 2020·Biochimica Et Biophysica Acta. Biomembranes·Jia BaiAyyalusamy Ramamoorthy
Oct 11, 2017·Journal of the American Chemical Society·Chih-Ta Henry ChienDaniel Nietlispach

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.