PMID: 20650093Jun 1, 1995Paper

Effects of mercury, methylmercury and aluminium on glial fibrillary acidic protein expression in rat cerebellar astrocyte cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
T A Toimela, H Tähti

Abstract

The effects of mercuric chloride, methylmercury chloride and aluminium chloride on glial fibrillary acidic protein (GFAP) expression in primary cerebellar astrocyte cultures were studied. GFAP has been found to be a quantitative marker of neuronal injuries on the central nervous system in vivo. The GFAP content of the astrocytes was examined by sandwich ELISA after exposure for 1 wk to various metal concentrations in the culture medium. Cellular injuries were assessed by quantifying the lactate dehydrogenase (LDH) leakage into the culture medium. The expression of GFAP was found at lower dose levels than the leakage of LDH, indicating that the expression of GFAP could be a more sensitive marker of neurotoxicity than LDH leakage. In morphological examination, staining with monoclonal antibody showed GFAP induction after mercury exposure.

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Citations

Apr 10, 2009·Cell Biology and Toxicology·Takeshi MinamiSeiji Ichida
Nov 29, 2007·Cellular and Molecular Neurobiology·Pooja Khanna, Bimla Nehru
Mar 25, 1998·Neurotoxicology and Teratology·R A Yokel, J P O'Callaghan
Aug 21, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Eduardo Luiz MoreiraMarcelo Farina
Nov 1, 2008·Environmental Toxicology and Pharmacology·Lindsay J Holden, Michael D Coleman
May 16, 2021·Molecular Neurobiology·Gabriela de Paula ArrifanoMaria Elena Crespo-Lopez

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