Effects of Metformin on Cellular Proliferation and Steroid Hormone Receptors in Patient-Derived, Low-Grade Endometrial Cancer Cell Lines.

Reproductive Sciences
Gretchen CollinsAnalisa DiFeo

Abstract

Endometrial cancer (EC) is the most common gynecologic malignancy and is the result of disruption of the balance between estrogen-stimulated growth and progesterone-induced growth modulation. Metformin has been shown to inhibit EC proliferation; however, its role in early-stage EC and its effects on steroid hormone receptors have not been adequately explored. Our aim was to examine the effects of metformin on cellular proliferation in patient-derived, low-grade EC cell lines and to determine whether it directly modulates steroid hormone receptor expression. Two novel EC cell lines were produced (EM2 and 3) from endometrial tumor tissue obtained from women undergoing surgery. Cellular proliferation was determined by the 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay, and in both cell lines, metformin decreased cell proliferation in a dose-dependent (10-200 µmol/L) manner and induced apoptosis as measured by cleaved PARP. Furthermore, metformin abrogated the effects of E2 on cell proliferation. Using quantitative real-time polymerase chain reaction and Western immunoblotting, metformin significantly decreased estrogen receptor (ER) α messenger RNA abundance but did not consistently affect the expression of pro...Continue Reading

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Citations

Feb 13, 2020·Reproductive Sciences·John Mark P PabonaRosalia Cm Simmen
Oct 20, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pamela T SolimanRobert L Coleman
May 29, 2021·Frontiers in Medicine·Emanuela Stochino-LoiJean Bouquet de Joliniere
Nov 11, 2021·Journal of Drug Targeting·Bartłomiej BarczyńskiJan Kotarski
Nov 18, 2021·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Jennifer Chae-KimClifford C Hayslip

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