Effects of metformin on mitochondrial function of leukocytes from polycystic ovary syndrome patients with insulin resistance

European Journal of Endocrinology
Victor M VictorAntonio Hernandez Mijares

Abstract

Oxidative stress and mitochondrial dysfunction are implicated in polycystic ovary syndrome (PCOS). The present study assesses the effect of metformin treatment on mitochondrial function in polymorphonuclear cells from PCOS subjects. Additionally, we evaluate endocrine parameters and levels of interleukin 6 (IL6) and tumour necrosis factor alpha (TNFα). Our study population was comprised of 35 women of reproductive age diagnosed with PCOS and treated with metformin for 12 weeks, and their corresponding controls (n=41), adjusted by age and BMI. We evaluated the alteration of endocrinological and anthropometrical parameters and androgen levels. Mitochondrial O2 consumption (using a Clark-type O2 electrode), membrane potential, mitochondrial mass, and levels of reactive oxygen species (ROS) and glutathione (GSH) (by means of fluorescence microscopy) were assessed in poymorphonuclear cells. H2O2 was evaluated with the Amplex Red(R) H2O2/Peroxidase Assay kit. IL6 and TNFα were measured using the Luminex 200 flow analyser system. Metformin had beneficial effects on patients by increasing mitochondrial O2 consumption, membrane potential, mitochondrial mass and glutathione levels, and by decreasing levels of reactive oxygen species and ...Continue Reading

References

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Citations

May 25, 2016·Annals of Clinical Biochemistry·Shouzhen ChenPing Long
Jul 7, 2017·Journal of Cellular Physiology·Silvia RaveraEnrico Cappelli
Feb 10, 2016·Oncotarget·Elisa PaolicchiStefano Landi
Aug 20, 2019·Reproductive Biology and Endocrinology : RB&E·Jingshun ZhangLianwen Zheng
Jun 24, 2017·Frontiers in Pharmacology·Maria A SortinoFilippo Drago
Aug 28, 2020·Journal of Cardiovascular Translational Research·Cevher OzcanZhenping Li
Oct 1, 2020·Trends in Pharmacological Sciences·Ling He
Mar 11, 2021·American Journal of Physiology. Cell Physiology·Leena P Bharath, Barbara S Nikolajczyk
Jul 3, 2021·International Journal of Molecular Sciences·Isabella PanfoliDavide Maggi
Nov 18, 2021·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Jenifer Lizbet Hernández-JiménezNayeli Torres-Ramírez

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