Effects of methotrexate on the tubal morphology of rabbits: evaluation by electron microscopy

Gynecologic and Obstetric Investigation
M Turan CetinIbrahim Urünsak

Abstract

To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge fine needle was used to puncture the right tubes of 10 rabbits in the study group. One month later, tubal biopsies were taken from both tubes of every rabbit in the study group. To mimic pregnancy, 100 IU hCG was injected intramuscularly to every rabbit 24 h before every surgery. On examination by electron microscopy, the effects of methotrexate and of the damage with the fine needle on the tubes were compared to the control group. Young epithelial cells of the methotrexate-injected fallopian tubes kept their normal structure, but others contained important ultrastructural changes. These were: nuclear pyknosis, cytoplasmic vacuolization, dilatation of endoplasmic reticulum cisternae, increased cytoplasmic density and compound cilia. Degenerative changes on epithelial cells caused by the temporary blockage of mitotic activity associated with local methotrexate injection are reversible through the formation of new epithelial cells.

References

Apr 1, 1987·Fertility and Sterility·D KenigsbergI M Spitz
Dec 1, 1988·British Journal of Obstetrics and Gynaecology·M AltarasN Ben-Aderet
Oct 1, 1989·American Journal of Obstetrics and Gynecology·C Egarter, P Husslein
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Jan 26, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·T KikuchiT Kobayashi
Jan 26, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·H HagiwaraK Takata

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Citations

Feb 12, 2014·Reproductive Toxicology·María Cecilia MartínGideon Koren
Jul 17, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Xiao-Jun YangFei-Yun Zheng
Jan 8, 2011·Cell Biochemistry and Function·Sritulasi Karri, Vanithakumari G
Sep 28, 2010·Fertility and Sterility·Xiao-Jun YangFei-Yun Zheng

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