Effects of methylmercury exposure on neuronal differentiation of mouse and human embryonic stem cells

Toxicology Letters
Xiaoming HeSeiichiroh Ohsako

Abstract

The establishment of more efficient in vitro approaches has been widely acknowledged as a critical need for toxicity testing. In this study, we examined the effects of methylmercury (MeHg), which is a well-known developmental neurotoxicant, in two neuronal differentiation systems of mouse and human embryonic stem cells (mESCs and hESCs, respectively). Embryoid bodies were generated from gathering of mESCs and hESCs using a micro-device and seeded onto ornithine-laminin-coated plates to promote proliferation and neuronal differentiation. The cells were exposed to MeHg from the start of neuronal induction until the termination of cultures, and significant reductions of mESCs and hESCs were observed in the cell viability assays at 1,10,100 and 1000nM, respectively. Although the mESC derivatives were more sensitive than the hESC derivatives to MeHg exposure in terms of cell viability, the morphological evaluation demonstrated that the neurite length and branch points of hESC derivatives were more susceptible to a low concentration of MeHg. Then, the mRNA levels of differentiation markers were examined using quantitative RT-PCR analysis and the interactions between MeHg exposure and gene expression levels were visualized using a net...Continue Reading

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Citations

Mar 16, 2016·Journal of Bioscience and Bioengineering·Hidenori TaniMasaki Torimura
May 3, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Shigeru YamadaYasunari Kanda
May 22, 2016·Nucleic Acids Research·Junko YamaneWataru Fujibuchi
Sep 7, 2017·Stem Cells and Development·Shuyu LiuFrancesco Faiola
Jul 10, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ellen FritscheJulia Tigges
Dec 12, 2018·Expert Opinion on Drug Metabolism & Toxicology·Ágota ApátiBalázs Sarkadi
Apr 13, 2019·Toxins·Slaven ErcegMª Ángeles Garcia-Esparza
Apr 9, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lisa M PrinceAaron B Bowman

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