PMID: 2501452Aug 1, 1989Paper

Effects of microdialysis-perfusion with anisoosmotic media on extracellular amino acids in the rat hippocampus and skeletal muscle

Journal of Neurochemistry
A Lehmann

Abstract

Changes in the levels of amino acids have been implicated as being important in osmoregulation both within and outside the CNS. The present study addressed the question of whether changes in osmolarity affect the extracellular concentration of amino acids in the rat hippocampus and femoral biceps muscle (FBM). Microdialysis probes were implanted in these tissues and perfused with standard physiological saline. Amino acid concentrations in the dialysate were determined with HPLC separation of o-phthaldialdehyde derivatives and fluorescence detection. The osmolarity of the perfusion buffer was gradually decreased by reduction of the concentration of NaCl from 122 to 61 to 0 mM. In other experiments, the osmolarity was increased by elevation of the NaCl level from 122 to 183 to 244 mM or by addition of mannitol. Glutamate, aspartate, gamma-aminobutyrate, and alanine levels in dialysate from the hippocampus increased when the concentration of NaCl was decreased by 61 mM, and they were further elevated when NaCl was omitted. Taurine and phosphoethanolamine (PEA) levels were maximally elevated at the intermediary decrease of NaCl concentration, and glutamine in particular but also methionine and leucine were suppressed by perfusion w...Continue Reading

References

Jul 1, 1977·Journal of Neurochemistry·P H Chan, R A Fishman
May 1, 1975·Journal of Neurochemistry·V J Balcar, G A Johnston
Apr 1, 1975·Journal of Neurochemistry·A Lajtha, H Sershen
Aug 1, 1986·Journal of Neurochemistry·J P PinJ Bockaert
Sep 1, 1986·Acta Physiologica Scandinavica·U Tossman, U Ungerstedt
Dec 1, 1987·Journal of Neuroscience Methods·K L RogersG R Dutton
Jan 1, 1988·Glia·H K Kimelberg, E O'Connor
Aug 15, 1988·Neuroscience Letters·J M SolísR Martín del Río
Jan 1, 1988·Life Sciences·M L NieminenM K Paasonen
Sep 1, 1988·Journal of Neurochemistry·J V WadeT L Pazdernik
Aug 1, 1987·Journal of Neurochemistry·J V WadeT L Pazdernik
Jan 1, 1987·Experimental Brain Research·W Walz, A F Allen
Jan 1, 1985·Journal of Neuroscience Research·M SandbergA Hamberger
Jul 1, 1968·Archives of Neurology·C G Wasterlain, R M Torack
Jul 1, 1968·Archives of Neurology·C G Wasterlain, J B Posner
Dec 18, 1981·Science·J H ThurstonE F Naccarato
Jul 15, 1980·The Journal of Comparative Neurology·J V NadlerC W Cotman
Sep 1, 1960·Journal of Neurochemistry·D R CURTIS, J C WATKINS
Jan 1, 1987·Neurochemistry International·A LehmannM Sandberg

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Citations

Oct 1, 1990·Annals of Neurology·A Lehmann
May 1, 1994·Journal of Neuroscience Research·Y KoyamaA Baba
Feb 15, 1995·Journal of Neuroscience Research·J E OlsonE M Ramnath
Nov 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·H Trachtman
Apr 1, 1994·Neurochemical Research·J MoránH Pasantes-Morales
Jun 1, 1996·Metabolic Brain Disease·S S Oja, P Saransaari
Oct 1, 1996·Molecular Neurobiology·S Basavappa, J C Ellory
Sep 1, 1991·Journal of Neuroscience Methods·A Bourdelais, P W Kalivas
Jan 1, 1990·Progress in Neurobiology·H Benveniste, P C Hüttemeier
Jun 17, 2000·Progress in Neurobiology·S S Oja, P Saransaari
Sep 7, 2000·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·E QuertemontP De Witte
Oct 17, 2008·American Journal of Physiology. Cell Physiology·Guangze Li, James E Olson
Oct 1, 1991·Journal of Internal Medicine·U Ungerstedt
Feb 2, 2008·Journal of Pharmaceutical Sciences·Jun ShenClinton F Stewart
Apr 27, 2011·Journal of Neurochemistry·María C Hyzinski-GarcíaAlexander A Mongin
Sep 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S SadoshimaM Fujishima
Jul 29, 1999·Biomedical Chromatography : BMC·C S Chaurasia
Dec 3, 1999·European Journal of Clinical Investigation·A GutierrezA Alvestrand
Jan 29, 2005·Acta Anaesthesiologica Scandinavica·C-J HuangJ W Skimming
May 1, 1991·Journal of Neurochemistry·A M Young, H F Bradford
Feb 16, 2006·Journal of Neurochemistry·James E Olson, Eduardo Martinho
Jan 12, 2007·American Journal of Physiology. Cell Physiology·Ingrid MoralesManuel Rodriguez
May 12, 1998·The American Journal of Physiology·J W Beetsch, J E Olson

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