Effects of Micronized Cartilage Matrix on Cartilage Repair in Osteochondral Lesions of the Talus.

Cartilage
Alvin K ShiehChristopher D Kreulen

Abstract

The repair of osteochondral lesions remains a challenge due to its poor vascularity and limited healing potential. Micronized cartilage matrix (MCM) is dehydrated, decellularized, micronized allogeneic cartilage matrix that contains the components of native articular tissue and is hypothesized to serve as a scaffold for the formation of hyaline-like tissue. Our objective was to demonstrate in vitro that the use of MCM combined with mesenchymal stem cells (MSCs) can lead to the formation of hyaline-like cartilage tissue in a single-stage treatment model. In group 1 (no wash), 250 µL MCM was reconstituted in 150 µL Dulbecco's phosphate-buffered saline (DPBS) for 5 minutes. Group 2 (saline wash) included 250 µL MCM washed in 20 mL DPBS for 30 minutes, then aspirated to remove all DPBS and reconstituted in 150 µL DPBS. Group 3 (serum wash): 250µL MCM washed in 20 mL DPBS for 30 minutes, then aspirated and reconstituted in 150 µL fetal bovine serum. Each group was then added to 50 µL solution of MSC suspended in DPBS at a concentration of 1.2 × 106 cells/350 µL. After 3 weeks, the defects were extracted and sectioned to perform viability and histologic analyses. Stem cells without rehydration of the MCM showed almost no viability wh...Continue Reading

References

Jan 6, 1999·The Journal of Bone and Joint Surgery. American Volume·J U YooB Johnstone
Apr 4, 2001·The Journal of Bone and Joint Surgery. British Volume·G I ImW H Cho
Oct 18, 2001·Clinical Orthopaedics and Related Research·J R SteadmanJ J Rodrigo
May 2, 2003·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·J Richard SteadmanWilliam G Rodkey
Mar 5, 2004·The Journal of Bone and Joint Surgery. American Volume·Gunnar KnutsenOddmund Johansen
Aug 24, 2005·Foot & Ankle International·Christoph Becher, Hajo Thermann
Sep 6, 2005·The Journal of Bone and Joint Surgery. American Volume·Kai MithoeferRobert G Marx
Apr 4, 2006·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Dae Kyung BaeSang Jun Song
May 3, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yiling LuFrancois Binette
Jun 1, 2006·The American Journal of Sports Medicine·Kai MithoeferRobert G Marx
Aug 20, 2010·The Journal of Bone and Joint Surgery. American Volume·Lisa A FortierAlan J Nixon
Jun 20, 2013·The Journal of Bone and Joint Surgery. American Volume·Christopher D Murawski, John G Kennedy
Sep 21, 2013·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Alberto GobbiAnup Kumar
Sep 10, 2014·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·Sarang Desai

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Citations

Aug 10, 2021·Foot and Ankle Surgery : Official Journal of the European Society of Foot and Ankle Surgeons·Sachin AllahabadiBrian C Lau

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