Effects of microRNA-183 on epithelial-mesenchymal transition, proliferation, migration, invasion and apoptosis in human pancreatic cancer SW1900 cells by targeting MTA1

Experimental and Molecular Pathology
Xizhou LinHaibo Yu

Abstract

This study aims to explore effects of miR-183 on epithelial-mesenchymal transition (EMT) and invasion by targeting MTA1 in human pancreatic cancer (PC) cells. Totally, 108 PC patients admitted in Wenzhou Central Hospital and Wenzhou People's Hospital, The Dingli Clinical Institute of Wenzhou Medical University from March 2010 to March 2014 were enrolled. qRT-PCR and immunohistochemistry were applied to examine expression of MTA1 mRNA and protein. Samples were divided into 6 groups: blank, NC, miR-183 mimics, miR-183 inhibitors, MTA1-siRNA and miR-183 inhibitors +MTA1-siRNA groups. CCK8 method was employed for determining cell proliferation rate, flow cytometry for cell apoptosis rate, scratch test for cell migration and Transwell assay for cell invasion. qRT-PCR and Western blotting were used to determine expression of MTA1, E-cadherin and Vimentin mRNA and protein. Positive expression rate of MTA1 was upregulated in PC tissues, and expression of miR-183 and MTA1 was associated with differentiation, migration, tumor size, TNM. The miR-183 mimics and MTA1-siRNA groups showed a decrease in proliferation, migration and invasion, whereas increased apoptosis, in comparison with blank and NC groups, as expression of MTA1 and Vimentin...Continue Reading

References

Jul 22, 2008·International Journal of Oral and Maxillofacial Surgery·G KawasakiA Mizuno
Apr 21, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Matthias D HoferVânia Nosé
Jun 9, 2009·Gastroenterology·Maria-Theresa MuellerChristopher Heeschen
Jul 9, 2009·Cancer Research·Thiruvengadam ArumugamWoonyoung Choi
Feb 4, 2010·Cancer Research·Yiwei LiFazlul H Sarkar
Apr 25, 2012·The American Journal of Pathology·Junfeng ZhuLiantang Wang
Aug 28, 2012·International Journal of Molecular Medicine·Haien ZhaoQingyu Fan
Oct 4, 2012·Journal of Cardiothoracic Surgery·Masashi TakemuraKayo Yoshida
Nov 10, 2012·Journal of Cellular Physiology·Shin HamadaTooru Shimosegawa
Apr 30, 2013·Gastroenterology·Dhiraj Yadav, Albert B Lowenfels
Oct 18, 2013·The New England Journal of Medicine·Daniel D Von HoffMarkus F Renschler
Oct 27, 2014·Cancer Metastasis Reviews·Nirmalya SenRakesh Kumar
Mar 17, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Mao-Wei ChengGu-Yu Hu
Dec 25, 2015·Journal of Experimental & Clinical Cancer Research : CR·Ning ZhouHaili Qian

❮ Previous
Next ❯

Citations

Sep 2, 2020·American Journal of Hypertension·Fu-Han GongWen-Lin Cheng
Sep 19, 2018·International Journal of Molecular Sciences·Eckhard KlieserDaniel Neureiter
Apr 8, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Yi HuangYuju Bai
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohadeseh FathiMohammad Taheri
Sep 26, 2021·Cell Death and Differentiation·Masahisa KudoCarlo M Croce

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.