Abstract
This study aimed to investigate the mechanism by which microRNA-206 (miR-206) affects the proliferation, apoptosis, migration and invasion of osteosarcoma (OS) cells by targeting ANXA2 via the AKT signaling pathway. A total of 132 OS tissues and 120 osteochondroma tissues were examined in this study. The targeting relationship between miR-206 and ANXA2 was verified with a dual-luciferase reporter assay. The miR-206 expression and ANXA2, AKT, PARP, FASN, Survivin, Bax, Mcl-1 and Bcl-1 mRNA and protein expression in the above two groups were examined by qRT-PCR and western blotting. The cultured OS cells were divided into 6 groups: a blank group, negative control (NC) group, miR-206 mimic group, miR-206 inhibitor group, si-ANXA2 group and miR-206 inhibitor + si-ANXA2 group. Cell cycle and apoptosis were assessed by flow cytometry, cell migration was examined with a wound-healing assay, and cell invasion was assessed with a Transwell assay. Pearson correlation analysis was used to determine the correlation between ANXA2 mRNA expression and miR-206 expression in OS. OS tissues exhibited increased mRNA and protein expression of ANXA2, AKT, PARP, FASN, Survivin, Mcl-1 and Bcl-2; decreased miR-206 expression; and decreased Bax mRNA an...Continue Reading
Citations
Nov 20, 2018·Journal of Cellular Biochemistry·Parastoo ShabaniBehzad Baradaran
Jan 23, 2019·Yonsei Medical Journal·Fang Biao ZhanQian Rong Deng
Oct 20, 2018·Journal of Cellular Biochemistry·Qian YuXian-Bo Peng
Apr 6, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G M VieraM S Brassesco
May 11, 2019·Cancer Medicine·Chunhua LingZeyi Liu
Apr 24, 2019·Aging and Disease·Qing HeZengqiang Yuan
May 31, 2019·Journal of Cellular Biochemistry·Shi-Qi ZhaoPing Han
Mar 8, 2019·Cardiovascular Research·Kristina SonnenscheinThomas Thum
Jan 3, 2020·Bioscience Reports·Yejun YanXiaodong Liu
Feb 14, 2020·Journal of Cellular Physiology·Yongkun WangJingzhe Zhang
Apr 15, 2020·Cell Cycle·Caifeng LiuJunhua Lu
Apr 12, 2020·Human Cell·Zhen GuoJunke Ge
Aug 5, 2020·Cell Biology International·Kaifee ArmanAhmet Arslan
Sep 10, 2020·Human Gene Therapy·Tiancheng LiShuifang Xiao
Jul 18, 2018·Molecular Medicine Reports·Jinhui LiuWenjun Cheng
Jun 19, 2019·Journal of Cellular Biochemistry·Congyi YangHongmei Wang
Dec 21, 2018·International Journal of Molecular Medicine·Yeletai NuerzhatiShan Zheng
Dec 20, 2018·Journal of Cellular and Molecular Medicine·Dan FeiKewei Zhang
Nov 18, 2018·Cancer Medicine·Ziyu ZhuYiwen You
Jul 30, 2020·PloS One·Alejandra Suarez-ArnedoCarolina Muñoz-Camargo
Jul 20, 2020·Cancer Letters·Hongliang ZhangWei Guo