Effects of microvirin monomers and oligomers on hepatitis C virus

Bioscience Reports
Yuan-Qin MinJosef Voglmeir

Abstract

Microvirin (MVN) is a carbohydrate-binding protein which shows high specificity for high-mannose type N-glycan structures. In the present study, we tried to identify whether MVN could bind to high-mannose containing hepatitis C virus (HCV) envelope glycoproteins, which are heavily decorated high-mannose glycans. In addition, recombinantly expressed MVN oligomers in di-, tri- and tetrameric form were evaluated for their viral inhibition. MVN oligomers bound more efficiently to HCV virions, and displayed in comparison with the MVN monomer a higher neutralization potency against HCV infection. The antiviral effect was furthermore affected by the peptide linker sequence connecting the MVN monomers. The results indicate that MVN oligomers such as trimers and tetramers may be used as future neutralization agents against HCV infections.

References

Dec 23, 2004·The Journal of Biological Chemistry·Toshiyuki MoriMichael R Boyd
Jun 16, 2005·Journal of Virology·Anne GoffardJean Dubuisson
Aug 19, 2005·Nature·Jordan J Feld, Jay H Hoofnagle
Jan 20, 2006·Molecular Microbiology·Jan-Christoph KehrElke Dittmann
Jul 1, 2006·The Journal of Biological Chemistry·François HelleJean Dubuisson
Jan 12, 2008·Journal of the American Society for Mass Spectrometry·Roxana E IacobKenneth B Tomer
Mar 4, 2008·Journal of Proteome Research·Alessio CeroniStuart M Haslam
Mar 18, 2008·Bioinformatics·Matthew P CampbellPauline M Rudd
Jan 27, 2009·Blood Purification·Richard H TullisVijay Kher
Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pei ZhangMarian Major
Jan 19, 2010·The Journal of Biological Chemistry·Michael D SwansonDavid M Markovitz
May 24, 2011·Virology Journal·Usman A AshfaqSheikh Riazuddin
Jul 20, 2011·Nature·Lauren Gravitz
Jul 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer R KeeffeStephen L Mayo
Sep 8, 2011·Antimicrobial Agents and Chemotherapy·Philip MeulemanJean Dubuisson
Nov 19, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Patrick R HarringtonLisa K Naeger
Nov 23, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Khayriyyah Mohd HanafiahSteven T Wiersma
Jul 10, 2013·Nature Medicine·T Jake Liang
Sep 17, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Florian DouamDimitri Lavillette
Jun 6, 2014·Nature·Charles M Rice, Mohsan Saeed
Sep 15, 2014·Journal of Hepatology·Stuart C GordonUNKNOWN HCV-TARGET study group
Nov 26, 2014·Journal of Pharmacology & Pharmacotherapeutics·Harmeet Kaur BhatiaNavreet Kaur Natt
Feb 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang LiuGong Cheng
Nov 10, 2015·Journal of Agricultural and Food Chemistry·Ya M DuLi Liu

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Citations

Oct 24, 2020·Glycobiology·Luís Cláudio Nascimento da SilvaMaria Tereza Dos Santos Correia
Feb 11, 2021·Marine Drugs·António PagareteVítor Verdelho
Apr 4, 2021·Biomolecules·Hanna Mazur-MarzecKrzysztof Pyrć

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Methods Mentioned

BETA
glycosylation
PCR
electrophoresis
ELISA
enzyme-linked immunosorbent assay
ion exchange purification

Software Mentioned

GlycoWorkbench
ImageJ

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