Effects of miR-199a on autophagy by targeting glycogen synthase kinase 3β to activate PTEN/AKT/mTOR signaling in an MPP+in vitro model of Parkinson's disease

Neurological Research
Rui-Qiong BaJun-Su Yang

Abstract

Objectives: miR-199a can regulate autophagy, its underlying mechanisms remain unknown. The purpose of this study was to investigate the mechanisms of miR-199a involved in regulating autophagy in a 1-methyl-4-phenylpyridine (MPP+)-induced in vitro model of PD.Methods: PC12 cells were incubated in MPP+, and the expression levels of miR-199a were bidirectionally regulated via either transfection of an miR-199a mimic or incubation in miR-199a inhibitors. The experimental manipulations were divided into four groups, including the control group, MPP+ group, MPP+ + miR-199a mimic group, and MPP+ + miR-199a inhibitor group. MTT, CCK-8, qRT-PCR, Western blotting and linear correlation analysis were performed to evaluate various experimental indicators.Results: At increasing MPP+ concentrations, the following results were found: the expression levels of miR-199a, phosphorylated AKT and mTOR proteins expression decreased; the expression levels of phosphatase and tensin homologue (PTEN), GSK3β, Beclin1, and LC3II increased; PC12 autophagy increased; and cellular viability and survival rates decreased. Transfection of an miR-199a mimic increased miR-199a expression and induced all of the following: the expression levels of PTEN, GSK3β, Becl...Continue Reading

References

Oct 18, 2005·Current Opinion in Cell Biology·Dos D SarbassovDavid M Sabatini
Jun 21, 2012·Biochemical and Biophysical Research Communications·Ning XuQiang Xia
Nov 26, 2013·Neurobiology of Aging·Ran XiongShengdi Chen
Jul 30, 2014·Frontiers in Cellular Neuroscience·Apostolia Fragkouli, Epaminondas Doxakis
Dec 9, 2014·Biochemical and Biophysical Research Communications·Dong-Mei ZhengJunji Ezaki
Apr 11, 2015·Nature Communications·Kun WangPei-Feng Li
Jul 15, 2015·Cell Death and Differentiation·Z LiX Yang
Feb 26, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aaron D ThomeDavid G Standaert
Apr 4, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ying LiuXiaotun Zhu
May 13, 2017·Current Neuropharmacology·Nicola TambascoPaolo Calabresi
Aug 23, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peifang LiuLihua Wang
Oct 18, 2018·European Journal of Pharmacology·Farshad Homayouni MoghadamMohammad Hossein Nasr-Esfahani
Feb 4, 2019·Molecular Genetics & Genomic Medicine·Maryam Talepoor ArdakaniKamran Ghaedi
Apr 8, 2019·Neuroscience Letters·Nikolaos PapagiannakisLeonidas Stefanis

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Citations

Jan 29, 2021·Molecular Biology Reports·Shunichi OkaUjjal K Bhawal
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Jan 30, 2021·Neural Regeneration Research·Sachchida Nand RaiBrijesh Kumar Singh
Jun 5, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ruili WangChen Li

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