Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia.

Molecular Medicine Reports
Caiyan HuLiu Fang

Abstract

GATA binding protein 1 (GATA‑1) is one of the most important hematopoietic transcription factors in the production of blood cells, such as platelets, eosinophils, mast cells and erythrocytes. GATA‑1 regulates the participation of microRNA (miRNAs/miRs) in erythroid differentiation under normoxia. However, GATA‑1 expression and the regulation of miR‑210‑3p in the context of erythroid differentiation under hypoxia remain unknown. The present study examined the expression levels of GATA‑1 and miR‑210‑3p in the model of erythroid differentiation in K562 cells under hypoxia, and determined the effects of GATA‑1, miR‑210‑3p and SMAD2 on erythroid differentiation through lentivirus transfection experiments. The present study detected increased GATA‑1 expression under hypoxia. Moreover, miR‑210‑3p was identified as a positive regulator of erythroid differentiation, which was upregulated both during erythroid differentiation and in GATA‑1 overexpression experiments under hypoxia. Importantly, in the K562 cell model of erythroid differentiation under hypoxia, miR‑210‑3p was upregulated in a GATA‑1‑dependent manner. Using a double luciferase reporter assay, miR‑210‑3p was identified as a downstream target of GATA‑1‑mediated regulation of ...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ShiozakiY Eto
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 29, 2002·Stem Cells·Yaron Shav-Tal, Dov Zipori
Jan 30, 2004·The EMBO Journal·Shingo MaedaKohei Miyazono
May 11, 2004·Veterinary Pathology·S H Tannehill-GreggM Weinstein
Jan 22, 2005·Seminars in Cell & Developmental Biology·John D Crispino
Feb 3, 2005·Molecular and Cellular Biology·Rita FerreiraSjaak Philipsen
Feb 3, 2005·British Journal of Haematology·A Daisy NarayanEsmail D Zanjani
May 6, 2005·Cytokine & Growth Factor Reviews·Kohei MiyazonoTakeshi Imamura
Mar 9, 2007·Postgraduate Medical Journal·Jeremy S Windsor, George W Rodway
Mar 21, 2007·Trends in Genetics : TIG·Timothy W Nilsen
Oct 16, 2007·Experimental Hematology·Heather M RogersConstance Tom Noguchi
Feb 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Louis C DoreMitchell J Weiss
May 22, 2008·British Journal of Haematology·Nobuyoshi KosakaTakashi Kato
May 24, 2008·Molecular Cell·William G Kaelin, Peter J Ratcliffe
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Clemens R ScherzerMichael G Schlossmacher
Aug 13, 2008·Developmental Cell·Jason E FishDeepak Srivastava
Jun 13, 2009·FEBS Letters·Yosuke MizunoYasushi Okazaki
Oct 3, 2009·Annals of the New York Academy of Sciences·Sofie Singbrant SöderbergStefan Karlsson
May 4, 2010·Trends in Molecular Medicine·Xin HuangAmato J Giaccia
May 7, 2010·American Journal of Physiology. Renal Physiology·Volker H Haase
Jun 2, 2010·The Journal of Experimental Medicine·Kasper D RasmussenDónal O'Carroll
Nov 26, 2010·The Journal of Experimental Medicine·Marc A Kerenyi, Stuart H Orkin
Feb 16, 2012·Epigenomics·Nicoletta BianchiRoberto Gambari
Apr 30, 2013·Blood Cells, Molecules & Diseases·Orawan SarakulTsukuru Umemura
Sep 24, 2013·Leukemia Research·Krystyna Mazan-Mamczarz, Ronald B Gartenhaus
Jan 24, 2014·Advances in Hematology·Ram Babu UndiRavi Kumar Gutti
Dec 15, 2015·Advances in Experimental Medicine and Biology·Sanjeev RaghuwanshiRavi Kumar Gutti
Aug 4, 2016·Molecular Medicine Reports·Youbang XieJianping Li
Dec 6, 2017·Acta Biochimica Et Biophysica Sinica·Fanglong WuXiao-Jing Wang
Nov 5, 2019·Differentiation; Research in Biological Diversity·Abdullah M AlMegbel, Charles F Shuler
May 1, 2020·Journal of Cellular and Molecular Medicine·Pedro A MartinezRajasekhar N V S Suragani

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