Effects of mismatch repair and Hpr1 on transcription-stimulated mitotic recombination in the yeast Saccharomyces cerevisiae

DNA Repair
Jennifer A Freedman, Sue Jinks-Robertson

Abstract

High levels of transcription driven by the GAL1-10 promoter stimulate mitotic recombination between direct repeats (DR) as well as between substrates positioned on non-homologous chromosomes. When the substrates are on non-homologous chromosomes, transcription stimulates both gene conversion and crossover events, but the degree of the stimulation varies depending on which substrate is highly transcribed. In gene conversion assays where only one of the substrates is highly transcribed, the effect of transcribing the donor versus the recipient allele can be highly asymmetric. We have examined the basis of this asymmetry and demonstrate that it relates to the nature of the mismatch present in recombination intermediates and the presence of the Msh3 mismatch repair (MMR) protein. In addition to examining the asymmetry conferred by donor versus recipient allele transcription, the possible contribution of transcription elongation problems to transcription-stimulated recombination has been examined using hpr1 mutants. Hpr1 is important for efficient elongation through certain sequences, and in hpr1 mutants, elongation problems have been correlated with elevated recombination between direct repeats. As expected, we found that combining...Continue Reading

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Citations

Oct 26, 2007·Molecular Genetics and Genomics : MGG·María García-RubioAndrés Aguilera
May 15, 2013·Journal of Bioscience and Bioengineering·Fahmida KhatunSatoshi Harashima
May 4, 2005·Yeast
Nov 26, 2014·The Biochemical Journal·Swarna Gowri ThotaRashna Bhandari
Nov 4, 2020·Cells·Nafiseh Chalabi Hagkarim, Roger J Grand

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