Effects of modifying the World Health Organization standard operating procedures for malaria microscopy to improve surveillance in resource poor settings

Malaria Journal
Sumadhya D FernandoRisintha G Premaratne

Abstract

Individuals with fever are screened for malaria in specially-established malaria diagnostic laboratories set up in rural hospitals in the Northern and Eastern Provinces of Sri Lanka. Large numbers of blood smears negative for malaria parasites are being screened daily. Good quality smears are essential to maintain a high diagnostic competency among the technical staff. The modifications made to the World Health Organization (WHO) standard operating procedures to improve the quality of smears have been studied. A blinded, controlled, interventional study was conducted in 22 intervention and 21 control malaria diagnostic laboratories. Changes were made to the WHO standard operating procedure protocols to prepare, stain and examine blood smears for malaria parasite detection which were implemented in intervention laboratories. These included wipe-cleaning slides, preparing both thick and thin smears on the same slide, reversing the order of collecting blood for thick and thin smears, dry fixing thick smear for 20-25 minutes under table lamp, polishing the edge of spreader slide with sand paper and fixing the thin smear with methanol if not stained within four hours. Parameters with respect to quality of the smear as per WHO criter...Continue Reading

References

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Citations

Apr 17, 2015·Tropical Medicine & International Health : TM & IH·Hans Van RemoortelSatya P Agarwal
Feb 13, 2016·Journal of Clinical Microbiology·Todd A. CuttsSteven S Theriault
Sep 6, 2018·Biomédica : revista del Instituto Nacional de Salud·Liliana Jazmín CortésMartha Stella Ayala
Apr 19, 2015·Malaria Journal·Natasha M LabanUNKNOWN Southern Africa International Centers of Excellence for Malaria Research
Jan 8, 2017·Malaria Journal·Juan CuadrosJose Miguel Rubio

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Methods Mentioned

BETA
light microscopy

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