Effects of Modulation of Ion Channel Currents by Salidroside in H9C2 Myocardial Cells in Hypoxia and Reoxygenation

Evidence-based Complementary and Alternative Medicine : ECAM
Xue-Bin CaoYang Li

Abstract

Salidroside, a phenyl-propanoid glycoside isolated from the medicinal plant Rhodiola rosea, has potent cardioprotective effects, especially against myocardial hypoxia and reoxygenation injury. However, the molecular mechanism underlying its action is still unclear. The aim of this study was to determine the effect of salidroside on sodium channel current (INa) and transient outward potassium channel current (Ito) in H9C2 cardiomyocytes. H9C2 cells were subcultured under anoxic conditions to mimic myocardial hypoxia and subsequently treated with salidroside. Whole cell patch clamp was performed to determine the effect of hypoxia/reoxygenation and salidroside on myocardial electrophysiological properties. In the differentiated H9C2 cells, hypoxia/reoxygenation reduced INa and Ito amplitude, while salidroside significantly restored both and altered the INa and Ito activation/inactivation kinetics in a dose-dependent manner. Our findings demonstrate that salidroside protects myocardial cells against hypoxia-reoxygenation by restoring the function of sodium and potassium channels.

References

Jul 1, 1996·The American Journal of Physiology·N Askenasy, G Navon
Jul 19, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Heinz GoegeleinHartmut Ruetten
Jan 6, 2012·The New England Journal of Medicine·Elizabeth G Nabel, Eugene Braunwald
Feb 20, 2013·Journal of Asian Natural Products Research·Mao-Chun XuHao Wang
Mar 29, 2013·European Heart Journal·Georg M FröhlichDerek J Hausenloy
Jan 24, 2014·Oxidative Medicine and Cellular Longevity·Yunru WangXuebin Cao

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Citations

Jun 11, 2020·Current Drug Metabolism·Sri Krishna Jayadev MaganiSrinivasan Tantravahi
Sep 2, 2021·Diabetic Medicine : a Journal of the British Diabetic Association·Weiwei HaoYuanyuan Yu

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