Effects of modulators of myosin light-chain kinase activity in single smooth muscle cells

Nature
T ItohF S Fay

Abstract

Phosphorylation of myosin light chains by a calmodulin-myosin light-chain kinase (MLCK) pathway is considered to be responsible for coupling increased calcium concentration with contraction in smooth muscle. This simple view has, however, recently been questioned. To test this hypothesis directly, we microinjected individual smooth muscle cells with modulators of the MLCK pathway while measuring contraction and calcium-ion concentration. Injection of a constitutively active proteolyzed form of MLCK causes contraction but no change in calcium concentration. By contrast, injection of peptide inhibitors of MLCK blocks contraction in response to K+ depolarization, despite the fact that the change in calcium concentration in response to stimulation was enhanced over controls. These results provide a direct demonstration at the level of a single cell that activation of the calmodulin-MLCK pathway is both necessary and sufficient to trigger contraction of smooth muscle.

References

Mar 1, 1977·The American Journal of Physiology·F S Fay, J J Singer
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Jul 1, 1987·The Journal of General Physiology·G J Kargacin, F S Fay
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S YagiF S Fay
Oct 1, 1987·The American Journal of Physiology·P L Becker, F S Fay
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F S Fay, C M Delise
Jan 1, 1982·Methods in Enzymology·F S FayP Merriam
Jan 1, 1983·Methods in Enzymology·M P WalshD J Hartshorne
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Aug 1, 1984·Pflügers Archiv : European journal of physiology·M J SiegmanA Michalek

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Citations

Feb 1, 2000·Journal of Cellular Physiology·R R Torgerson, M A McNiven
Jan 1, 1990·Cell Motility and the Cytoskeleton·K A Giuliano, D L Taylor
Jan 1, 1991·Cell Motility and the Cytoskeleton·K M Trybus
Jun 15, 1994·Molecular and Cellular Biochemistry·M P Walsh
Nov 1, 1993·Molecular and Cellular Biochemistry·R S MoussaviR S Adelstein
Jan 1, 1992·Pharmacology & Therapeutics·J M Chalovich
Jun 1, 1992·Brain Research. Molecular Brain Research·A M EdelmanJ Cho-Lee
Dec 1, 1992·Neurochemistry International·R J Colbran
May 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R A MurphyJ D Strauss
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G PfitzerJ M Chalovich
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W WalkerF S Fay
Oct 7, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Elisha R InjetiWilliam J Pearce
Jun 1, 1991·Hypertension·J T StullK E Kamm
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·A YoshiiM Mori
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·U MalmqvistF S Fay
Aug 1, 1990·American Journal of Obstetrics and Gynecology·H IzumiK Shirakawa
Aug 11, 2010·Experimental Physiology·J Graham McGeown
Jan 22, 2005·European Journal of Pharmacology·Li Chu, Masao Endoh
Feb 1, 1991·Current Opinion in Cell Biology·J R Sellers
Sep 27, 2005·Biochimica Et Biophysica Acta·Marie A BogoyevitchAlbert J Ketterman
May 29, 1992·Biochemical and Biophysical Research Communications·S MorelandR S Moreland
Sep 24, 1998·Biophysical Journal·R A Tuft, L M Loew

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