Effects of molecular weight of hyaluronic acid on its viscosity and enzymatic activities of lysozyme and peroxidase

Archives of Oral Biology
Jihoon KimHong-Seop Kho

Abstract

To investigate the effects of the molecular weight of hyaluronic acid on its viscosity and enzymatic activities of lysozyme and peroxidase in solution and on the hydroxyapatite surface. Hyaluronic acids of four different molecular weights (10 kDa, 100 kDa, 1 MDa, and 2 MDa), hen egg-white lysozyme, bovine lactoperoxidase, and human whole saliva were used. Viscosity values of hyaluronic acids were measured using a cone-and-plate viscometer at six different concentrations (0.1-5.0 mg/mL). Enzymatic activities of lysozyme and peroxidase were examined by hydrolysis of fluorescein-labeled Micrococcus lysodeikticus and oxidation of fluorogenic 2',7'-dichlorofluorescein to fluorescing 2',7'-dichlorofluorescein, respectively. In solution assays, only 2 MDa-hyaluronic acid significantly inhibited lysozyme activities in saliva. In surface assays, hyaluronic acids inhibited lysozyme and peroxidase activities; the inhibitory activities were more apparent with high-molecular-weight ones in saliva than in purified enzymes. The 100 kDa-hyaluronic acid at 5.0 mg/mL, 1 MDa-one at 0.5 mg/mL, and 2 MDa-one at 0.2 mg/mL showed viscosity values similar to those of human whole saliva at a shear rate range required for normal oral functions. The diff...Continue Reading

Citations

Jul 25, 2019·Journal of Drug Targeting·Marialucia GalloriniLeonardo Osti
Jul 3, 2019·International Journal of Molecular Sciences·Dawid ŁysikJoanna Mystkowska
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicola d'AvanzoMassimo Fresta
Jul 3, 2021·Biomechanics and Modeling in Mechanobiology·Peter A TorzilliIsabella L Pecorari

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