Effects of monensin liposomes on the cytotoxicity, apoptosis and expression of multidrug resistance genes in doxorubicin-resistant human breast tumour (MCF-7/dox) cell-line

The Journal of Pharmacy and Pharmacology
Madhu Sudhan ShaikM Singh

Abstract

We have evaluated the effects of monensin liposomes on drug resistance reversal, induction of apoptosis and expression of multidrug resistance (MDR) genes in a doxorubicin-resistant human breast tumour (MCF-7/dox) cell line. Monensin liposomes were prepared by the pH-gradient method. MCF-7/dox cells were treated with various anticancer drugs (doxorubicin, paclitaxel and etoposide) alone and in combination with monensin liposomes. The cytotoxicity was assessed using the crystal violet dye uptake method. The induction of apoptosis in MCF-7/dox cells was assessed by established techniques such as TUNEL (terminal deoxynucleotidyl transferase-mediated nick end labelling) staining and caspase-3 assay. The effect of monensin liposomes on doxorubicin accumulation in MCF-7/dox cells was monitored by fluorescent microscopy. Finally, the expression of MDR genes (MDR1 and MRP1) in MCF-7/dox cells following the exposure to doxorubicin alone and in combination with monensin liposomes was evaluated by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Our results indicated that monensin liposomes overcame drug resistance in MCF-7/dox cells to doxorubicin, etoposide and paclitaxel by 16.5-, 5.6- and 2.8-times, respecti...Continue Reading

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Citations

Oct 17, 2012·Journal of Veterinary Pharmacology and Therapeutics·G-Y WangS-X Jiang
Dec 17, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Wojciech CybulskiWojciech Rzeski
Aug 6, 2019·Advanced Pharmaceutical Bulletin·Rehmat Jan, Gul-E-Saba Chaudhry
Nov 21, 2019·Drug Delivery·Yuzhu SunShuyu Xie
May 19, 2007·Journal of Medical Microbiology·Jorge Castro-GarzaLucio Vera-Cabrera
Apr 14, 2005·Molecular Cancer Therapeutics·Danica RamljakRobert B Dickson

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