PMID: 6165249May 1, 1981Paper

Effects of monensin on amylase release from mouse parotid acini

The American Journal of Physiology
E L WatsonW Farnham

Abstract

Isolated mouse parotid acinar cells (acini) were prepared by enzyme digestion, divalent cation depletion, and mechanical shearing. Acini were found to be morphologically intact, i.e., 95% viable as judged by trypan blue exclusion. Amylase release by the cholinergic agonist carbachol, by the beta-adrenergic agonist isoproterenol, and by monensin was similar to responses obtained in mouse parotid fragments. Monensin-stimulated amylase release was associated with enhanced 22Na+ uptake and 45Ca2+ efflux; monensin did not affect 45Ca2+ uptake. In the absence of extracellular Na+, the response to monensin (50 microM) was reduced from 162 +/- 33.5 to 12.4 +/- 0.5%; monensin also failed to stimulate 45Ca2+ efflux. Similar results were obtained with isoproterenol (10(-6) M). The results suggest that Na+ ions may play a role in amylase release possibly by releasing Ca2+ from internal stores.

References

Sep 17, 1979·Life Sciences·B A LeslieJ W Putney
Sep 1, 1975·The American Journal of Physiology·J A MangosT Barber
Dec 15, 1976·The Biochemical Journal·P Kanagasuntheram, P J Randle
Nov 1, 1976·The American Journal of Physiology·J A WilliamsB Moffat
Aug 1, 1974·Journal of Molecular and Cellular Cardiology·E CarafoliC Kratzing
Oct 15, 1967·Experientia·H RinderknechtB J Haverback
Jun 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·J H HANKS, R E WALLACE

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Citations

Jun 1, 1989·The Journal of Membrane Biology·M F MarianiG D van Rossum
Feb 1, 1984·Journal of Dental Research·E WatsonH Horwitz
Feb 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·V Nassar-Gentina, M Luxoro
Nov 8, 1982·Life Sciences·E L WatsonF Dowd
Jun 15, 1987·Biochimica Et Biophysica Acta·T Takuma, T Ichida
Oct 1, 1993·Molecular and Cellular Endocrinology·T Fridolf, B Ahrén
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·H Takemura, H Ohshika
Apr 1, 1984·Cell Calcium·R M Case
Jan 1, 1987·Archives of Oral Biology·J R Martinez, S Barker
Jan 1, 1988·Archives of Oral Biology·J R MartinezP Reed
May 30, 1989·Biochemical and Biophysical Research Communications·K W SnowdowneJ R Cashman
Feb 28, 1983·Biochemical and Biophysical Research Communications·E L Watson, F J Dowd

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