Effects of monofilament nylon coated with basic fibroblast growth factor on endogenous intrasynovial flexor tendon healing

The Journal of Hand Surgery
Yoshitaka HamadaNatsuo Yasui

Abstract

We developed a monofilament nylon thread that can release various growth factors to enhance intrinsic reparative processes after flexor tendon injury. We evaluated the properties of this thread in vitro and in vivo. Nylon threads were coated with gelatin that subsequently was cross-linked in glutaraldehyde. The thread was soaked in basic fibroblast growth factor (bFGF) solution (400 microg/mL). Exogenous bFGF in the thread was released constantly over the course of 1 week. The biologic activity of bFGF and the biomechanical strength of the thread were examined in vitro and its efficacy was investigated in an in vivo rabbit tendon repair model after early flexion exercises. The sutured sites were examined histologically (hematoxylin-eosin, immunohistochemistry, in situ hybridization), biochemically (Western blot test), and biomechanically (ultimate load) after surgery. This gelatin-coated thread absorbed iodine 125-labeled bFGF in a time-dependent manner. The total amount of bFGF absorbed by the thread within the tendon tissue was between 3 and 15 mug depending on the concentration of bFGF solution. Basic fibroblast growth factor protein was delivered selectively-not in the surrounding scar but in the repaired tendon-for 3 weeks...Continue Reading

References

Jan 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B A AlmanH J Wolfe
Dec 1, 1996·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·U KhanD A McGrouther
Jun 18, 1999·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·N KusanoY Maki
Jul 22, 1999·Experimental Cell Research·G Serini, G Gabbiani
Nov 30, 1999·Plastic and Reconstructive Surgery·R J RohrichH H Orenstein
Aug 16, 2002·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Matthew J SilvaRichard H Gelberman
Apr 17, 2003·Sports Medicine·Timothy MolloyGeorge Murrell
Jul 14, 2004·The Journal of Hand Surgery·Charles Hsu, James Chang
Jun 1, 2005·The Journal of Hand Surgery·Stavros ThomopoulosRichard H Gelberman

❮ Previous
Next ❯

Citations

Sep 21, 2010·British Medical Bulletin·Umile Giuseppe LongoVincenzo Denaro
Oct 12, 2010·The Journal of Bone and Joint Surgery. American Volume·Stavros ThomopoulosRichard H Gelberman
Dec 3, 2014·Advanced Drug Delivery Reviews·Denitsa DochevaChristopher H Evans
Jun 23, 2012·The Journal of Hand Surgery·Daisuke SatoToshihiko Ogino
Jun 21, 2011·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·Ali Moshiri, Ahmad Oryan
Dec 10, 2008·Clinics in Sports Medicine·Lawrence V Gulotta, Scott A Rodeo
Mar 5, 2016·ACS Applied Materials & Interfaces·Smrithi PadmakumarDeepthy Menon
Jun 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stavros ThomopoulosRichard H Gelberman
Jan 8, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jin Bo TangPaul Y Liu
Jun 13, 2012·Journal of Tissue Engineering and Regenerative Medicine·A Oryan, A Moshiri
Nov 6, 2010·The Journal of Hand Surgery·R Frank HennScott W Wolfe
Aug 1, 2015·Sports Medicine and Arthroscopy Review·Knut BeitzelAugustus D Mazzocca
Oct 31, 2014·Tissue Engineering. Part a·Connie S ChamberlainWilliam L Murphy
Jan 10, 2014·Foot & Ankle International·Samuel B AdamsLew C Schon
Jun 22, 2012·Surgical Innovation·Dénes Balázs HorváthyZsombor Lacza
Oct 29, 2013·The Journal of Hand Surgery, European Volume·O A BranfordK J Rolfe
Dec 22, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephen W LindermanRichard H Gelberman
Sep 28, 2010·Surgical and Radiologic Anatomy : SRA·Tarik CavusogluIbrahim Tekdemir
Aug 7, 2018·Hand : Official Journal of the American Association for Hand Surgery·Kunihide MuraokaJeffrey Yao
Mar 4, 2011·The Journal of the American Academy of Orthopaedic Surgeons·David E RuchelsmanMichael E Rettig
Sep 10, 2013·Journal of Orthopaedic Surgery·Zafar AhmadNeil Rushton
Mar 13, 2021·Journal of Hand and Microsurgery·Yoshitaka MinamikawaYoshitaka Hamada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.