PMID: 6413655Nov 1, 1983Paper

Effects of monovalent and divalent cations on Ca2+ fluxes across chromaffin secretory membrane vesicles

Journal of Neurochemistry
H Krieger-Brauer, M Gratzl

Abstract

Bovine chromaffin secretory vesicle ghosts loaded with Na+ were found to take up Ca2+ when incubated in K+ media or in sucrose media containing micromolar concentrations of free Ca2+. Li+- or choline+-loaded ghosts did not take up Ca2+. The Ca2+ accumulated by Na+-loaded ghosts could be released by the Ca2+ ionophore A23187, but not by EGTA. Ca2+ uptake was inhibited by external Sr2+, Na+, Li+, or choline+. All the 45Ca2+ accumulated by Na+-dependent Ca2+ uptake could be released by external Na+, indicating that both Ca2+ influx and efflux occur in a Na+-dependent manner. Na+-dependent Ca2+ uptake and release were only slightly inhibited by Mg2+. In the presence of the Na+ ionophore Monensin the Ca2+ uptake by Na+-loaded ghosts was reduced. Ca2+ sequestered by the Na+-dependent mechanism could also be released by external Ca2+ or Sr2+ but not by Mg2+, indicating the presence of a Ca2+/Ca2+ exchange activity in secretory membrane vesicles. This Ca2+/Ca2+ exchange system is inhibited by Mg2+, but not by Sr2+. The Na+-dependent Ca2+ uptake system in the presence of Mg2+ is a saturable process with an apparent Km of 0.28 microM and a Vmax = 14.5 nmol X min-1 X mg protein-1. Ruthenium red inhibited neither the Na+/Ca2+ nor the Ca2+/...Continue Reading

References

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Citations

Jan 1, 1986·Biochimica Et Biophysica Acta·D NjusG J Harnadek
Oct 5, 2011·Cellular and Molecular Life Sciences : CMLS·Enrico Zampese, Paola Pizzo
Jan 20, 1986·FEBS Letters·F U Reiffen, M Gratzl
Jan 1, 1988·Progress in Histochemistry and Cytochemistry·C Heym, W Kummer
Mar 23, 2010·Trends in Cell Biology·Sandip Patel, Roberto Docampo
Jul 1, 1991·Cell Calcium·D A PowisH R Von Grafenstein
Aug 1, 1989·Journal of Neurochemistry·H R von Grafenstein, D A Powis
May 3, 2005·Cell Calcium·Alfredo MorenoJavier Alvarez
Oct 14, 2011·The Biochemical Journal·Anthony J MorganAntony Galione
May 24, 2011·Biochimica Et Biophysica Acta·Natalia DionisioJuan A Rosado
Dec 17, 1985·Biochemistry·D Bulenda, M Gratzl
Jan 1, 1987·Annals of the New York Academy of Sciences·H WinklerM Schober
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Apr 15, 1996·Annals of the New York Academy of Sciences·A S Schneider, C R Jan

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